超声快速制备病理切片中的超声频率优化  

Optimization of ultrasound frequencies in the ultrasound method for quickly preparing pathological sections

作　　者：陈谦[1] 邹欣晔[1] 袁忆丰[1] 程建春[1] 

机构地区：[1]南京大学声学研究所近代声学国家重点实验室,南京210093

出　　处：《声学学报》2008年第2期171-177,共7页Acta Acustica

基　　金：国家杰出青年科学基金(10125417)

摘　　要：报告了利用超声增强细胞膜通透性的作用实现超声快速制备病理切片的方法。实验比较了超声处理和常规处理两种方法制取的肝等病理组织切片,比较了在优化频率范围内选择200 kHz,400 kHz,600 kHz,800 kHz和1000 kHz五组超声频率作用相同肝组织样品制备病理切片的效果。结果表明超声方法可以在1h内完成肝组织切片,且品质较好,同时发现超声作用频率不同,完成肝组织处理的总时间不同,200 kHz和800 kHz频率完成肝组织处理的时间最短。引入液体环境中微气泡空化的理论模型,依据该模型数值模拟得到的最佳超声作用频率与实验结果一致。The ultrasound rapid tissue processing method was applied successfully to high-speed preparation of the pathological sections through the effect that the ultrasound can enhance the cell membrane permeability. The experimental results by comparing two types of liver tissue pathological sections, which were processed respectively with conventional processing method and the ultrasound processing method with 200 kHz, 400 kHz, 600 kHz, 800 kHz and 1000 kHz frequencies in the optimization frequency range, showed that the processing of liver tissue with the ultrasound processing method is accomplished in 1 hour, and that the processing times of the same liver samples with different ultrasound frequencies are different, the liver tissue processing times with 200 kHz and 800 kHz frequencies are shorter. A theoretical model was presented based on microbubble cavitation in the liquid environment, and the numerical simulations based on this model found that the optimal frequencies of the ultrasound rapid tissue processing are consistent with the experimental results.

关 键 词：超声处理 频率优化 病理切片 快速制备 病理组织切片 细胞膜通透性 实验比较 超声作用 

分 类 号：R446.8[医药卫生—诊断学]