HPLC测定中药成分溶度参数的理论与实验研究  

Theoretical and experimental studies on solubility parameters of multiple components for traditional Chinese materia medica with HPLC

作　　者：贺福元[1] 周宏灏[1] 罗杰英[2] 邓凯文[3] 扶瑾[2] 皮凤娟[2] 吴德智[1] 戴儒文[1] 

机构地区：[1]中南大学临床药理研究所,湖南长沙410078 [2]湖南中医药大学药学院,湖南长沙410208 [3]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《中国中药杂志》2008年第6期642-648,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金(30572378)

摘　　要：目的:创立HPLC测定中药成分溶度参数的理论与方法。方法:运用色谱理论及H ildebrand-Scatchard溶解度理论创立HPLC法测定化合物的溶度参数的数学表达式,用已知溶度参数的咖啡因验证该理论并对大黄类成分进行测定。HPLC条件,色谱柱为A lltech Apollo C18柱(4.6 mm×250 mm,5μm);流动相为乙腈-水,梯度洗脱;检测波长咖啡因270 nm,大黄类成分254 nm;柱温25℃;进样体积10μL。结果:建立了保留时间与溶度参数关联的数学表达式,用此测得咖啡因的溶度参数为28.31 J1/2.cm-3/2,与文献28.84 J1/2.cm-3/2基本一致,用此法测得芦荟大黄素、大黄酸、大黄素、大黄素甲醚的溶度参数依次为39.70 J1/2.cm-3/2,39.08 J1/2.cm-3/2,38.37 J1/2.cm-3/2,36.42 J1/2.cm-3/2。结论:HPLC法能同时测定多个成分的溶度参数,这为研究中药的配伍溶解规律,单味中药剂改工艺研究奠定理论基础。Objective： To establish the theories and methods to determine solubility parameters of multiple components for the traditional Chinese material medica （TCMM） with HPLC. Method： The mathematical expresses to determin the solubility parameters were established according to chromatographic and Hildebrand-Scatchard theories, The HPLC experiments were carried out at 40 ℃ on an Alltech Apollo C18 column （4. 6 mm×250 mm, 5 μm） eluted with acetone and water in gradient mode. The flow rate was 1. 0 mL·min^-1, and the detection wavelength was 254 nm. The injecttion volume was 1 0 μL. Result： The mathematical expresses between the retention time and the solubility parameters were established and used to determin caffeine solubility parameter as 28. 31 J^1/2·cm^-3/2 in accordance with 28. 84 J^1/2·cm^-3/2 reported by literature, and those of aloe-emodin, rhein, emodin, physcione as 39. 70 J^1/2·cm^-3/2, 39. 08 J^1/2·cm^-3/2, 38. 37 J^1/2·cm^-3/2, 36. 42 J^1/2·cm^-3/2 respectively. Conclusion： The retention time of HPLC can be used to determine the solubility parameters of multiple componets. The established method is useful for the compatibility rule study of traditional Chinese medicine.

关 键 词：溶度参数 咖啡因 芦荟大黄素 大黄酸 大黄素 大黄素甲醚 

分 类 号：R284[医药卫生—中药学]