44种中药中1,2,3,4,6-五-O-倍酰-D-葡萄糖含量的测定  

Determination of 1,2,3,4,6-penta-O-galloyl-D-glucose in forty four kinds of Chinese traditional medicines by HPLC

作　　者：王维聪[1] 王潮[1] 宋学英[1] 赵文华[1] 王桥[1] 

机构地区：[1]首都医科大学化学生物学与药学院,北京100069

出　　处：《中国中药杂志》2008年第6期656-659,共4页China Journal of Chinese Materia Medica

基　　金：北京市属市管高等学校人才强教计划资助项目;首都医科大学科研基金(2006ZR09)

摘　　要：目的：采用高效液相色谱测定44种中药中1,2,3,4,6-五-O-倍酰-D-葡萄糖（1,2,3,4,6-penta-O-galloyl-D-glucose，PGG）的含量。方法：以乙醇-水溶液（50∶50）为提取剂，在50～60 ℃的温度下超声1 h，从中药中提取PGG；采用Diamonsil C18（4. 6 mm×250 mm，5 μm）色谱柱，流动相为乙腈-2. 5 ％醋酸水溶液（18∶82），紫外检测波长280 nm，流速1. 0 mL·min^-1。结果：PGG在1～150 μg·mL^-1呈良好的线性关系（r = 0. 999 4）。五倍子、牡丹皮、赤芍、白芍等17种中药中均含有PGG，且在五倍子、牡丹皮、赤芍、白芍中PGG含量较高。结论：本实验用简单快捷的方法考察了44种中药中PGG的含量，并准确测定了五倍子等17种含PGG中药中PGG的含量，为从中药中分离、纯化PGG以及中药的开发利用提供了实验基础。Objective： To determine 1, 2, 3, 4, 6-penta-O-galloyl-D-glucose （PGG） in forty four kinds of Chinese traditional medicines by HPLC. Method： The PGG was extracted with a solvent consisting of ethanol and water （50∶50） in ultrasonic bath at 50-60 ℃ for 1 hour. A Diamonsil C18 column （4. 6 mm×250 mm, 5 μm） was used for the separation and analysis of PGG with a mobile phase consisting of acetonitrile and 2. 5 % acetic acid solution （18∶82）. The wavelength of detection was at 280 nm, and flow rate was set at 1. 0 mL·min^-1. Result： The calibration curve for PGG is linear over the range of 1-150 μg·mL^-1 （r=0. 999 4）, and PGG was found in seventeen kinds of Chinese traditional medicines, such as gall tree peony bark, red peony root, white peony root,and so on. Conclusion： The contents of PGG were determined in forty four kinds of Chinese traditional medicines by a rapid and precision HPLC method.

关 键 词：1 2 3 4 6-五-O-倍酰-D-葡萄糖 高效液相色谱法 中药提取 

分 类 号：R284.1[医药卫生—中药学]