磷钼蓝光度法测定磷酸钙中的磷含量  

Determination of phosphorus content in calcium phosphate by phosphomolybdate blue spectrophotometry

作　　者：李素芹[1] 张军[1] 王俊凤[1] 宋邦才[1] 王志锋[1] 

机构地区：[1]河南科技大学化工与制药学院,河南洛阳471003

出　　处：《无机盐工业》2008年第3期55-56,60,共3页Inorganic Chemicals Industry

基　　金：河南省科技攻关项目(0524240014);河南科技大学重大科技预研专项项目(2004ZD005)

摘　　要：以二氯化锡-甘油混合液作还原剂，建立了用磷钼蓝分光光度法测定生物陶瓷基材β-磷酸钙中磷含量的方法，确定了最佳测定条件。在波长为690nm处，磷的质量浓度在0～0．4μg／mL时符合比耳定律，线性回归方程为y=0．0153x-0．0003，相关系数R^2=0．9973，表观摩尔吸光系数为2．55×10^4 L／（mol·cm），相对标准偏差在0．09％～0．12％，加标回收率98．72％以上，方法的精密度和准确性均较好。Using tin bichloride - glycerol mixed solution as reductant, the determination method of phosphorus content for bioceramic base - material -β - calcium phosphate with phosphomolybdate blue spectrophotometry has been established. The optimum determination conditions were confirmed：at the wavelength of 690 rim, Beer＇s law was obeyed in the mass concentration range of phosphorus from 0 to 0.4 pμg/mL;the linear regression equation is y = 0. 015 3 x - 0. 000 3; the correlation coefficient R^2 is 0. 997 3 ; and its apparent molar absorptivity is 2.55 x 10^4 L/（ mol .cm ） ; RSD is in the range of 0.09% - 0.12% and the standard sample recovery rate is over 98.72%. The method is feasible both in precision and in accuracy.

关 键 词：分光光度法测定 磷含量 磷酸钙 磷钼蓝 表观摩尔吸光系数 线性回归方程 相对标准偏差 加标回收率 

分 类 号：TQ153.12[化学工程—电化学工业] S816.17[农业科学—饲料科学]