HPLC法测定静态加压溶剂提取马兜铃属植物中马兜铃酸  

HPLC Determination of aristolochic acids in plants of Aristolochia L.by static pressurized liquid extraction

作　　者：梁庆优[1] 郑颖[1] 唐海谊 李绍平[1] 王一涛[1] 

机构地区：[1]澳门大学中华医药研究院,中国澳门 [2]澳门理工学院高等卫生学校,中国澳门

出　　处：《中草药》2008年第3期364-368,共5页Chinese Traditional and Herbal Drugs

基　　金：澳门科学技术发展基金资助项目(049/2005/A-R1)

摘　　要：目的建立并优化从马兜铃药材中提取马兜铃酸和的静态加压溶剂提取方法,考察各因素对提取效率的影响,并测定马兜铃、关木通、青木香和广防己中两成分的量。方法以马兜铃酸和的量为指标,采取单因素考察的方法,分别优化提取溶剂、药材粒径、提取温度、提取压力、提取时间、冲洗体积、循环数目、药材加入量等影响因素。结果优化后的条件为:甲醇提取,药材粉末粒径100～120目,提取温度120℃,提取压力10.3MPa,静态提取时间10min,冲洗体积40%,1个循环,药材加入量1.00g。结论本法可一次将马兜铃酸和从马兜铃等4种药材中完全提取,显示了良好的适用性。经比较,该法比传统的超声和索氏提取方法提取效率更高,节约溶剂,省时省力;静态加压溶剂法更适合于青木香中马兜铃酸的提取。Objective To optimize static pressurized liquid extraction （PLE） method for the extraction of aristolochic acids Ⅰ and Ⅱ （AAⅠ and AA Ⅱ ） from Fructus Aristolochiae and study the influences of re- lated factors. Methods The univariate design was introduced. The operational parameters, such as the type of solvent, particle size of the sample powder, extraction temperature, pressure, static time, flush volume, the number of cycles, and the amount of sample were optimized. Results The optimized result employed methanol as extraction solvent, particle size of 100--120 meshes, extraction temperature of 120 ℃, extraction pressure of 10.3 MPa, static time of 10 min, flush volume of 40%, 1 cycle, and sample amount of 1.00 g. The method was applied for four species of traditional Chinese medicinal materials including Fructus Aristolochiae, Caulis Aristolochiae Manshuriensis, Radix Aristolochiae, and Radix Arsitolochiae Fangchi. Conclusion This method can be used to completely extract AA Ⅰ and AA Ⅱ from Fructus Aristolochiae in once extraction. The comparison shows that this static PLE method is better than ultrasonication and Soxhlet methods with higher extraction efficiency and less time-consuming. It is also better than the dynamic one in the extraction of AAs from Radix Aristolochiae.

关 键 词：马兜铃 关木通 青木香 广防己 马兜铃酸 静态加压溶剂提取 高效液相色谱 

分 类 号：R284.2[医药卫生—中药学] R286.02[医药卫生—中医学]