人、大鼠烧伤皮肤提取物抗原特性的研究  

The antigenic specificity of burned skin extracts of both human and rat

作　　者：贾晓明[1,2,3] 朱兆明[1,2,3] 李成文 孔秋华[1,2,3] 于燕[1,2,3] 王亚平 秦勤[1,2,3] 

机构地区：[1]解放军304医院烧伤整形科 [2]军事医学科学院五所 [3]解放军304医院创伤研究室

出　　处：《军医进修学院学报》1997年第2期81-83,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：国家自然科学基金

摘　　要：为了从抗原分子水平上分析与研究人与大鼠烧伤皮肤提取物ＨＢＳＥ与ＨＮＳＥ，以及人与大鼠新鲜皮提取物（ＲＢＳＥ与ＲＮＳＥ）之间的异同，本实验采用双相免疫扩散、单相免疫扩散、火箭免疫电泳、抗体ＩｇＧ的纯化与ＨＲＰ标记，以及ＥＬＩＳＡ等方法的免疫化学分析。结果表明，ＨＢＳＥ和ＲＢＳＥ的抗原性强于ＨＮＳＥ与ＲＮＳＥ的抗原性及抗ＨＢＳＥ和抗ＲＢＳＥ的特异性，与ＨＮＳＥ和ＲＮＳＥ仅有很弱的交叉反应。因而提示，ＨＢＳＥ和ＨＮＳＥ，ＲＢＳＥ和ＲＮＳＥ之间抗原性有较大的差别，而并不是一种物质。A series of experiments was carried out to investigate the nature and the difference between the normal and burned skin extracts from human (HNSE and HBSE) and rat (RNSE and RBSE). Immunochemical analysis including immuno diffusion test, rocket immunoelectrophoresis, and ELISA were performed. The results showed that:(1) the antigenicity of burned skin was stronger than that of normal skin;(2)the extracts of burned skin composed of a very high specificity as compared with the normal skin and there was very little crossreactivity between them. Our data indicate that the antigenicity of HNSE and HBSE as well as that of RNSE and RBSE is quite different, and thus these substances may be very discrepant in nature.

关 键 词：烧伤 皮肤 免疫电泳 抗原 

分 类 号：R644[医药卫生—外科学]