表达轮状病毒SA11株Vp4的抗原表位诱导病毒中和抗体生成  

Induction of Neutralizing Antibodies by SAll Vp4-Specific Epitopes

作　　者：陈元鼎[1] 刘名英[1] 邹永健 彭梅 戴长柏[1] FranciscoE.Baralle 

机构地区：[1]中国医学科学院

出　　处：《中国病毒学》1997年第2期125-131,共7页Virologica Sinica

基　　金：云南省科委研究基金

摘　　要：以昆虫病毒Ｆｌｏｃｋｈｏｕｓｅｖｉｒｕｓ（ＦＨＶ）外壳蛋白为载体的外源抗原表位表达系统（ＦＨＶ－ＲＮＡ２载体系统），在重组杆状病毒和重组ｐＥＴ系统中构建和表达了ＳＡ１１Ｖｐ４胰酶切割位点两则和重叠切割位点３个抗原表位氨基酸序列（抗原表位Ａ，ａａ２２３－２４２；抗原表位Ｂ，ａａ２４３－２６２，抗原表位，Ｃａａ２３４－２５１）并对其原免疫原性进行了研究。结果表明。In the present study, with a new foreign epitope - presenting system, FHV - RNA2 carrier system, on the precursor capsid protein of an insect virus, flock house virus (FHV) as a carrier, in recombinant baculovirus system and in pET system three epitopes derived from the amino acid residues surrounding the trypsin cleavage site(s) of SAll Vp4 (epitope A, aa 223 to 242;epitope'B, aa 243 to 262; and epitope C, aa 234 to 251 ) was constructed and expressed. The ability of the three epitopes inducing neutralizing antibodies in animals was studies. The results showed that these three epitopes induced production of seral antibodies and neutralizing anbodies in guinea pigs. These findings suggested that the amino acid sequences surrounding the trypsin cleavage site (s) on SAll Vp4 have great significance in the development of epitope - based recombinant ro-tavirus subunit vaccines.Keywoeds: Rotavirus Vp4, Trypsin cleavage site, Epitopes, Neutralizing antibodies, FHV RNA2 carrier system

关 键 词：轮状病毒Vp4 抗原表位 中和抗体 研制 

分 类 号：R373.25[医药卫生—病原生物学] R392－33[医药卫生—基础医学]