树类-细小病毒的初步研究  

Preliminary Report of Parvovirus-like Virus in Tupaia belangeri (Tree Shrew)

作　　者：吴小闲[1] 蒋虹[1] 涂新明[1] 张新生[2] 施慧君[1] 

机构地区：[1]中国医学科学院中国协和医科大学实验动物研究所,北京100021 [2]成都军区后勤部军事医学研究所,昆明650032

出　　处：《中国病毒学》1997年第2期167-170,共4页Virologica Sinica

摘　　要：从树肾细胞培养物中分离出3株病毒，嗜在对数分裂期的TL细胞上复制，产生细胞病变和血凝素抗原，能凝集豚鼠和小鼠红细胞。交互血凝抑制实验表明3株病毒同属一个血清型。免疫酶染色显示抗原首先出现于细胞核。电镜观察负染标本病毒形态近似圆形和六角形，无胞膜，直径约30nm。血清学检查大多数树血清中含有该病毒抗体，证明是树的一种潜在病毒。初步鉴定为类细小病毒。Three viruses were isolated from the kidney cell culture of Tupaia belangeri (tree shrew).They would like to replicate in mitosis of TL cells, to produce CPE and hemagglutinin and to hemaggulate the erythrocytes of guinea pig and mouse. The viruses belong to one serum type by hemagglutination inhibition test. The virus antigen appeared first at the nuclei by immunoenzymatic stain. The morphology of the virus was similar to round and hexagonal without capsule, diameter 30 nm by negative stain under electron microscope. Most number of sera in tree shrews appeared the virus antibody by serological assay. It was proved that the virus was a latent virus in tree shrews and identified preliminarily as parvo- like virus.

关 键 词：类-细小病毒 树QU 

分 类 号：R373.9[医药卫生—病原生物学]