β_2-Adrenoceptor affinity chromatography and its application in the screening of the active compounds from Semen Armeniacae Amarum  

作　　者：ZHENG XiaoHui ZHAO XinFeng YANG Rong WANG ShiXiang WEI YinMao ZHENG JianBin 

机构地区：[1]Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Institute of Analytical Science, Northwest University, Xi'an 710069, China [2]Engineering and Research Center of Chinese Herb Modemization, Xi'an Jiaotong University, Xi'an 710061, China

出　　处：《Chinese Science Bulletin》2008年第6期842-847,共6页

基　　金：the National Natural Science Foundation of China (Grant No. 20575052);the Natural Science Foundation of Shaanxi Province, China (Grant Nos. 2006B03 and 2006C220)

摘　　要：β2-Adrenoceptor (β2-AR) was purified from the rabbit lung tissue by sepharose-salbutamol affinity chromatographic column. To prepare the β2-AR stationary phase, β2-AR was evenly immobilized on the surface of macro-pore silica with a mild chemical coupling method through covalent bond. The reten- tion properties of β2-AR stationary phase were characterized by four ligands, salbutamol sulfate, noradrenaline bitartrate, adrenaline hydrochloride and propranolol hydrochloride, to establish the β2-AR affinity chromatography. Then, the method was used to screen the active compounds from the total extracts of Semen Armeniacae Amarum. The results showed that β2-AR on the surface of the sta- tionary phase could keep its original bioactivity and selectivity. Amygdalin retained in the chroma- tographic column was proved to be the active compound of the total extracts of Semen Armeniacae Amarum. Compared with the existing chromatographic screening approaches, this method showed a good stability and high selectivity. The active compounds which could interact with β2-AR in traditional Chinese medicine (TCM) could be screened efficiently by this method, providing a new way to screen the active compounds in complicated samples such as TCM.β2-Adrenoceptor （β2-AR） was purified from the rabbit lung tissue by sepherose-salbutamol affinity chromatographic column. To prepare the β2-AR stationery phase, β2-AR was evenly immobilized on the surface of macro-pore silica with e mild chemical coupling method through covalent bond. The retention properties of β2-AR stationery phase were characterized by four ligends, selbutamol sulfate, noredreneline bitartrete, adrenaline hydrochloride end proprenolol hydrochloride, to establish the β2-AR affinity chromatography. Then, the method was used to screen the active compounds from the total extracts of Semen Armeniacae Amarum. The results showed that β2-AR on the surface of the stationary phase could keep its original bioectivity end selectivity. Amygdalin retained in the chromatographic column was proved to be the active compound of the total extracts of Semen Armeniacae Amarum. Compared with the existing chromatographic screening approaches, this method showed e good stability end high selectivity. The active compounds which could interact with β2-AR in traditional Chinese medicine （TCM） could be screened efficiently by this method, providing e new way to screen the active compounds in complicated samples such as TCM.

关 键 词：苦杏仁 生物活性 亲合色谱法 配体 

分 类 号：R284.1[医药卫生—中药学]