中国部分地区铜绿假单胞菌β-内酰胺酶基因型研究  

Genotype distribution of β-lactamases produced by Pseudomonas aeruginosa in some areas of China

作　　者：王春新[1] 糜祖煌[2] 黄支密[3] 钱小毛[4] 常东[5] 李智山[6] 

机构地区：[1]南京医科大学附属无锡第一医院,无锡214002 [2]无锡市克隆遗传技术研究所,无锡214026 [3]解放军98医院,湖州313000 [4]绍兴第二人民医院,绍兴312000 [5]解放军304医院,北京100037 [6]同济医学院附属襄樊医院,襄樊441021

出　　处：《中国抗生素杂志》2008年第3期164-167,共4页Chinese Journal of Antibiotics

基　　金：南京医科大学科技发展基金资助项目(NY0584);无锡市克隆遗传技术研究所专项基金资助项目(20050102WX)

摘　　要：目的调查中国部分地区临床分离铜绿假单胞菌β-内酰胺酶(β-lactamases,BLAs)基因型分布。方法收集北京、浙江、江苏及湖北等地区5家医院临床分离的165株铜绿假单胞菌,PCR及测序方法检测10种BLAs。结果165株铜绿假单胞菌114株检出BLAs的基因,总检出率为69.10%,其中TEM、CARB、OXA、PER、VIM、IMP、GES、DHA型的检出率分别为41.21%、15.15%、13.94%、6.67%、5.45%、4.85%、4.85%和3.03%,未检出SHV型和VEB型BLAs的基因。19株(16.81%)同时产两种BLAs的基因,12株(10.62%)同时产3种BLAs的基因。各地区BLAs的基因型有较大差别。结论中国部分地区铜绿假单胞菌携带BLAs的基因型主要是TEM型,部分菌株同时产两种及两种以上BLAs的基因;各地区BLAs的基因型有较大差别。Objective To investigate the genotype distribution of β-lactamases（BLAs） produced by Pseudomonas aeruginosa in some areas of China. Methods One hundred and sixty-five clinical strains of Pseudomonas aeruginosa were collected from five hospitals in different areas. PCR amplifying and DNA sequencing were carried out. Results Of 165 isolates, 114 were positive for BLAs genes（69. 10% ） and the positive rates of genes of TEM, CARB, OXA, PER, VIM, IMP, GES and DHA were 41.21% , 15. 15% , 13.94%, 6.67% , 5.45% , 4.85%, 4.85% and 3.03% ,respectively, the genes of SHV and VEB were all negative. Nineteen isolates（16.81% ） carried two types of BLAs genes and twelve isolates（10.62% ）carried three types of BLAs genes. Conclusion This study showed that TEM type was the most common genotype in BLAs produced by Pseudomonas aeruginosa in China and some isolates produced two or three types of BLAs. The genotype distribution of BLAs produced by Pseudomonas aeruginosa was discrepant in the different areas of China.

关 键 词：铜绿假单胞菌 Β-内酰胺酶 基因型 

分 类 号：R378.991[医药卫生—病原生物学] R512.62[医药卫生—基础医学]