4株红螺菌科细菌的拮抗作用及其影响因素  

Antagonistic effects of four strains of Rhodospirillaceae and factors affecting inhibitory function

作　　者：邱宏端[1] 徐姗楠[1] 蔡玉婵[1] 杨泳芝 谢航[1] 

机构地区：[1]福州大学生物科学与工程学院,福州350002

出　　处：《农业工程学报》2008年第2期194-198,共5页Transactions of the Chinese Society of Agricultural Engineering

基　　金：福建省自然科学基金(B0210004);福建省海洋与渔业局资助项目(闽海渔科0411号);福建省科技厅资助项目(2005N038)

摘　　要：对4株红螺菌(荚膜红假单胞菌PSB-2、球形红假单胞菌PSB-3、胶质红假单胞菌H1和沼泽红假单胞菌H10)的去细胞上清液(CFS)抑制水产养殖病原菌的作用进行研究,并探讨养殖水体环境对红螺菌拮抗作用的影响。结果表明,4株红螺菌在细胞生长稳定期后能有效分泌拮抗物质,其去细胞上清液(CFS)对20多株水产养殖病原菌均有抑制作用。从抑菌活性看,荚膜红假单胞菌的抑菌活性强于其他菌株:荚膜红假单胞菌对水产养殖病原菌的最低抑菌浓度(MIC)为8～64 AU/mL,而其他红螺菌株多为4～16 AU/mL。在养殖水体的生态因子中,水体pH、NH4+-N与NO2--N对红螺菌代谢产物的抑菌活性影响较大,pH7～9,NH4+-N低于5 mg/L、NO2--N浓度低于7mg/L时抑菌活性较强,而养殖水体温度与溶氧变化对红螺菌的抑菌活性基本无影响作用。The inhibitory effects of cell-free supernatant （CFS） produced by four strains of Rhodospirillaceae （Rps.capsulata PSB-2, Rps.sphaeroides PSB-3, Rps.gelatinosa H1 and Rps.palustris H10） against pathogenic bacterium were assayed. And the influences of aquaculture environmental factors on antagonistic effects of metabolic products were studied. The results show that antagonistic materials of four strains of RhodospiUaceae were secreted after the stationary phase, and cell-free supernatant （CFS） had inhibitory ability against more than twenty pathogenic bacteria. As for inhibitory activity, Rps.capsulata had the strongest antibacterial activity among four strains of Rhodospirillaceae, and its MIC against pathogenic bacteria was 8～ 64AU/mL. MIC of other three strains of Rhodospirillaceae were 4～16AU/mL. Antibacterial activities of RhodospiriUaceae were affected significantly by pH value and the concentrations of NH4^＋-N and NO2^--N： antimicrobial effects were greatly significant between pH 7 and pH 9, and under the concentrations of NH4^＋-N below 5 mg/L and NO2^--N below 7 mg/L, but antimicrobial activities were not sensitive to the changes of temperature and Dissolved Oxygen （DO） in aquaculture water.

关 键 词：红螺菌 水产养殖病原菌 拮抗作用 环境因子 

分 类 号：X52[环境科学与工程—环境工程]