魔芋软腐病拮抗菌BJ-1的鉴定  

Identification of Antagonistic Bacteria BJ-1 against Soft Rot of Konjak

作　　者：马琼[1] 

机构地区：[1]生物资源保护与利用湖北省重点实验室湖北民族学院生物科学与技术学院,湖北恩施445000

出　　处：《安徽农业科学》2008年第5期1936-1936,1943,共2页Journal of Anhui Agricultural Sciences

基　　金：国家科技攻关计划项目(2003BA901A05)

摘　　要：[目的]为魔芋软腐病的生物防治提供理论基础。[方法]采用平板对峙培养法从健、病魔芋植株及其根际土壤中进行拮抗菌的初筛和复筛,对筛选出的魔芋软腐病拮抗菌BJ-1进行形态学观察和生理生化鉴定。[结果]从发病植株中分离获得的BJ-1对魔芋软腐病有较强的拮抗作用,拮抗带宽度达10 mm。复筛试验表明,内生拮抗菌BJ-1具有广谱抗菌活性,对软腐病原菌的拮抗作用最强,拮抗带宽度达12 mm。形态学观察、生理生化试验发现BJ-1与芽孢杆菌属细菌的形态学特征和生理生化性状相似,初步认为魔芋软腐病拮抗菌BJ-1为芽孢杆菌属细菌。[结论]魔芋软腐病拮抗菌BJ-1为芽孢杆菌属细菌,对软腐病原菌有强拮抗作用。[Objective] The study aimed to supply theoretical foundation for the biological control on the soft rot of konjak, [Method] The primary and secondary screening were conducted on the antagonistic bacteria from healthy and diseased konjak plants and their rhizosphere soils by confronting plate culture and the morphological observation and physiological and biochemical identification were conducted on the selected antagonistic bacteria BJ-1 against soft rot of konjak. [Result] The BJ-1 separated fresh diseased plant had stronger antagonistic action on the soft rot of konjak with the width of antagonistic strip reaching 10 mm. The secondary screening experiment showed that entophytic antagonistic bacteria BJ-1 had broad-spectrum antibacterial activity and strongest antagonistic action on the pathogen of soft rot with the width of antagonistic strip reaching 12 ram. It was found in the morphological observation and physiological and biochemical experiments that the morphological characters and physiological and biochemical properties of BJ-1 were similar to that of Bacillus. It was preliminarily thought that antagonistic bacteria BJ-1 against soft rot of konjak belonged to Bacillus. [Conclusion] The antagonistic bacteria BJ-1 against soft rot of konjak belonged to Bacillus and had strong antagonistic action on the pathogen of soft rot.

关 键 词：魔芋 软腐病 拮抗 鉴定 

分 类 号：S435.3[农业科学—农业昆虫与害虫防治]