分子印迹液相色谱整体柱的制备研究  

Study on the preparation of monolithic imprinted HPLC column

作　　者：左国强[1] 张裕平[2] 龚文君[2] 张毅军[2] 李全民[1] 

机构地区：[1]河南师范大学化学系,河南新乡453003 [2]河南科技学院,河南新乡453003

出　　处：《化学试剂》2008年第3期175-177,共3页Chemical Reagents

基　　金：中韩国际合作协议项目(20611140646);河南省高校新世纪优秀人才项目(2006HNCET-01)资助

摘　　要：以对羟基苯甲酸为模板,丙烯酰胺为功能单体,乙二醇二甲基丙烯酸酯为交联剂,偶氮二异丁腈为热引发剂原位制备了液相色谱分子印迹整体柱,羟基苯甲酸的异构体可获得基线分离,而空白柱没有印迹效果。实验同时对分子印迹过程的机理进行了探讨。A monolithic molecularly imprinted polymer with specific recognition ability for 4-hydroxybenzoic acid （4-HBA） was prepared by in-situ thermal polymerization, using aerylamide as the functional monomer, ethylene glycol dimethacrylate （EDMA） as the cross-linking agent, toluene and dodeeanol as porogenic solvents and azobisisobutyronitrile （AIBN） as the thermal polymerization initiator. Baseline separation of isomers of hydroxybenzoic acid was achieved on the prepared HPLC imprinted column, but not on the blank polymer. The basic principle and mechanism of molecular imprinting polymers were discussed in this paper.

关 键 词：分子印迹聚合物 整体柱 液相色谱 

分 类 号：O657.7[理学—分析化学]