β-CM-7对MCF-7、SKOV_3和SK-N-SH肿瘤细胞增殖和凋亡的影响  

Effects of β-casomorphin-7 from β-casein on the proliferation and apoptosis of MCF-7,SKOV_3 and SK-N-SH cells

作　　者：周艳[1] 秦宜德[1] 董华胜[1] 顾芳[1] 

机构地区：[1]安徽医科大学生物化学与分子生物学教研室,合肥230032

出　　处：《安徽医科大学学报》2008年第1期5-9,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省教育厅自然科学基金项目(编号:2005JK257)

摘　　要：目的探讨β-酪啡肽-7（β-CM-7）在体外对人乳腺癌细胞（MCF-7）、人卵巢癌细胞（SKOV3）和人神经母细胞瘤细胞（SK—N—SH）增殖和凋亡的影响。方法用MTT测定法观察β-CM-7在体外对MCF-7、SKOV3、SK—N—SH细胞增殖的影响。用流式细胞仪测定β-CM-7在体外对MCF-7和SKOV3细胞凋亡的影响。结果β-CM-7作用于MCF-7细胞24和48h，对其增殖影响不明显（P〉0．05）。2．5×10^-6 -2．5×10^-2g／L的β-CM-7作用72h，能抑制MCF-7细胞增殖（P〈0．05或P〈0．01），抑制率为17．50％～32．08％。β-CM-7作用MCF-7细胞72h，在2．5×10^-7～2．5×10^-2g／L剂量下，诱导肿瘤细胞的凋亡（P〈0．05或P〈0．01），凋亡率为9．23％～29．41％，而在作用48h后，仅在2．5×10^-3g／L的高浓度下具有诱导肿瘤细胞的凋亡作用，凋亡率为10．12％。β-CM-7作用于SKOV3细胞24～72h，只有在高剂量（2．5×10^-2g／L和／或2．5×10^-3g／L）时，抑制肿瘤细胞增殖（P〈0．05），抑制率为10．50％～19．44％。β-CM-7作用SKOV、细胞48h和72h，在2．5×10^-3～2．5×10^-2g／L高剂量下能诱导肿瘤细胞的凋亡（P〈0．05或P〈0．01），凋亡率为8．72％～17．83％。β-CM-7对SK—N—SH肿瘤细胞增殖影响无显著性（P〉0．05）。结论β-CM-7能够抑制MCF-7和SKOV3细胞增殖，诱导细胞凋亡，作用结果与其剂量和作用时间有关。β-CM-7对SK—N—SH细胞的增殖无明显影响。Objective To study the effects of β-casomorphin-7 （ β-CM-7 ） on the proliferation and apoptosis of human breast cancer cell lines （ MCF-7 ）, human ovarian cancer cell lines （ SKOV3 ） and human neuroblastoma cell lines （SK-N-SH） in vitro. Methods Inhibitions of β-CM-7 on MCF-7, SKOV3 and SK-N-SH cell proliferation, in vitro, were investigated by MTT colorimetric assay. Apoptosis percentages of MCF-7 and SKOV3 cells induced with β-CM-7, in vitro, were measured by flow cytometric assay （FCM）. Results β-Casomorphin-7 had no significant effect on the proliferation of MCF-7 cell cultured in 24 h and 48 h, but β-CM-7 at 2.5 ×10^ -6- 2.5 ×10^ -2 g/L significant inhibited MCF-7 cell proliferation in 72 h （ P 〈 0. 05 or P 〈 0. 01 ）, inhibitory rate of which were 17.50% - 32.08%. Cultured in 72 h, β-CM-7 at 2.5 ×10^ -7 - 2.5 × 10^-2 g/L notably induced MCF-7 apoptosis （P 〈0. 05 or P 〈0. 01 ）, its apoptosis rate being 9. 23% -29. 41%. If cuhured in 48h, only β-CM-7 at high dose （2.5 ×10^-3 g/L） could induce MCF-7 apoptosis （P 〈0.05）. For SKOV3, no matter what cultured at 24 h, 48 h or 72 h, β-CM-7 at high dose（2. 5× 10^-3 and/or 2. 5 × 10^-2 g/L） could inhibit cell proliferation and induce cell apoptosis （P 〈0.05 or P 〈 0. 01 ）, their inhibitory and apoptosis rate were 10. 50% - 19.44% and 8.72% - 17.83%, respectively. However, it wasn＇t distinct for the influence of β-CM-7 on SK-N-SH cell proliferation. Conclusion β-CM-7 could inhibit proliferation and induce apoptosis in MCF-7 and SKOV3, the effects depend on β-CM-7 dose and test time. β-CM-7 affects SK-N-SH very little.

关 键 词：内啡肽类 乳腺肿瘤/病理学 神经母细胞瘤/病理学 细胞凋亡 细胞分裂 

分 类 号：R341.6[医药卫生—基础医学] R971.9