3种海藻的粗蛋白对植物病原真菌的抑制作用  

Inhibition of Plant Pathogenic Fungi by Crude Proteins from Three Marine Algae

作　　者：陈国强[1] 郑怡[1] 林勇[2] 刘艳如[1] 

机构地区：[1]福建师范大学生命科学学院,福建福州350108 [2]福建省农业科学院土壤肥料研究所,福建福州350013

出　　处：《福建师范大学学报（自然科学版）》2008年第2期67-70,共4页Journal of Fujian Normal University：Natural Science Edition

基　　金：福建省自然科学基金资助项目(B0510008);福建省科技厅重点项目(2005N036)

摘　　要：以圆弧青霉(Penicillium cyclopium)、绿色木霉(Trichoderma viride)、红色链孢霉(Neurcsporacrassa)、玉米大斑病菌(Helminthosporium turcicum)、稻瘟病菌(Piricularia oryzae)和黄曲霉(Aspergillusflavus)等6种植物病原真菌为供试菌,检测了3种海藻(铁钉菜Ishige okamurae、羊栖菜Sargassum fusiforme、冈村凹顶藻Laurencia okamurai)的粗蛋白对它们的菌丝生长及孢子萌发的影响.实验表明,3种海藻的粗蛋白对6种真菌的菌丝生长及孢子萌发的作用效果不同,其中冈村凹顶藻粗蛋白对红色链孢霉菌丝的生长,羊栖菜粗蛋白对稻瘟病菌孢子的抑制作用最明显,而羊栖菜和铁钉菜粗蛋白对红色链孢霉的菌丝生长,冈村凹顶藻粗蛋白对绿色木霉及黄曲霉的孢子萌发均无抑制效果.3种海藻的粗蛋白能凝集兔血红细胞,表明它们当中含有凝集素.Crude proteins from three marine algae were obtained with ammonium sulfate and assayed for inhibition against six phytopathogenic fungi, Penicillium cyclopium, Trichoderma viride, Neurcspora crassa, Helminthosporium turcicum, Piricularia oryzae, Aspergillus flavus. The result showed that crude proteins from three marine algae could have different inhibition to hyphal growth or spore germination of fungi tested. Strong inhibitions were found in crude proteins of Laurencia okamurai against hyphal growth of Neurcspora crassa. The crude proteins of Sargassum fusiforme also have strong inhibition against Piricularia oryzae. No inhibition was found in crude proteins of Sargassurn fusiforrne and Ishige okarnurae against hyphal growth of Neurcspora crassa, and in Laurencia okarnurai against spore germination of Trichoderrna viride and Aspergillus flavus. Crude proteins of three marine algae could agglutinate rabbit erythrocytes, which indicated these crude proteins contain lectins.

关 键 词：海藻 粗蛋白 植物病原真菌 菌丝生长 孢子萌发 

分 类 号：Q946[生物学—植物学] Q949.2