利用蜘蛛酯酶检测蔬菜中的有机磷农药残留  

Detection the organophosphorous pesticide residues in vegetables using the esterase of Araneus ventricosus (Araneae)

作　　者：刘红海[1] 邹晶[1] 张春学 匡毅[3] 彭宇[1] 

机构地区：[1]湖北大学生命科学学院,湖北武汉430062 [2]山东省东营市河口农业局,山东东营257200 [3]江汉大学化学与环境工程学院,湖北武汉430056

出　　处：《湖北大学学报（自然科学版）》2008年第1期76-78,共3页Journal of Hubei University：Natural Science

基　　金：国家自然科学基金(No:30270895);湖北省教育厅基金重大项目(No:Z200710002)资助

摘　　要：探讨利用大腹园蛛酯酶检测蔬菜中有机磷农药残留的技术和方法.结果表明:大腹园蛛腹部的酯酶活性显著高于头胸部,在37℃和缓冲液pH为7.0时,酯酶活性最高.有机磷农药乐果浓度的对数值与酶抑制率存在较好的直线关系,通过检测蔬菜洗脱液对蜘蛛酯酶抑制率的方法可以用来检测蔬菜中的有机磷农药残留.采用该方法检测时,乐果的检出限为0.5094 mg/L.受检3种蔬菜的有机磷农药残留量均低于规定的标准.A technique for detection of organophosphorous（Ops） pesticide residues was explored by measuring the activities of esterase from Araneus ventricosus. The results were showed that the esterase activities in abdomen from the adult female spider were higher than that in cephalothorax. The esterase activity was the highest under 37℃ and with pH 7. 0 of phosphate buffer solution. There was a significant liner regression between the concentration logarithm of dimethoate and the inhibitory rate of esterase. The Ops pesticide residues can be detected by measuring the inhibitory rate of vegetables washing liquid to the esterase from the spider. The detection limit of dimethoate was 0. 509 4 mg/L by this method. The Ops pesticide residues in three species of vegetables were lower than the standard values given by Chinese government and FAO/WHO.

关 键 词：大腹园蛛 酯酶 有机磷农药残留 

分 类 号：Q656.21[生物学—生物物理学]