针刺大鼠不同穴位诱发的后三里区域外周传入神经信号特征研究  

Study on Nerve Discharges of Peripheral Afferent Innervated ST36 Evoked by Manual Acupuncture at Different Acupoints in Rats

作　　者：李为民[1] 陈颖波 王晓艳 丁光宏[1] 

机构地区：[1]复旦大学力学与工程科学系,上海200433 [2]上海市针灸经络研究中心,上海201203

出　　处：《上海针灸杂志》2008年第3期39-42,共4页Shanghai Journal of Acupuncture and Moxibustion

基　　金：上海市科委课题资助项目(05DZ19747;06DZ19732);国家重点基础研究发展计划973课题(2005CB523306);上海市重点学科建设项目(B112)

摘　　要：目的用记录支配后三里穴位区域的神经束放电方法,观察手针刺激大鼠后肢后三里、三阴交以及腹部天枢穴位后,诱发该神经束的放电变化,为揭示针刺信息的外周传导规律,提供研究方法和依据。方法在分离支配大鼠右侧后三里穴位神经束(坐骨神经分支)后,分别以手针刺激同侧的后三里、三阴交及天枢穴(捻转提插,1 Hz),记录并分析神经束诱发放电;然后注射神经阻滞药利多卡因封闭右侧后三里穴位,观察上述针刺诱发神经放电的变化。结果手针刺激后三里可诱发明显的神经束放电,同样手针刺激三阴交、天枢穴则无上述神经的放电现象。利多卡因封闭后三里穴位后,手针刺激后三里,此诱发放电随之消失;而用生理盐水进行同样的注射后上述手针刺激的诱发放电依然存在。结论手针刺激后三里穴位可诱发支配穴位的坐骨神经分支特异性神经放电,提示针刺后三里信息可沿该分支传入脊髓。Objective To investigate the characteristic of peripheral nerve discharges evoked by manual acupuncture at different acupoints in anesthetized rats. Methods Acupoints of Housanli （ ST 36）, Sanyinjiao （ SP 6） and Tianshu （ ST 25） were stimulated by twisting an inserted needle from left to right combined with up and down movement at a rate of 1 Hz. A branch of sciatic nerve innervated ST 36 was dissected for recording electrical discharges. Lidocaine was injected for blockading nerve innervation. Results There were significant nerve discharges recorded from the afferent innervated ST 36 when applying a manual acupuncture at ST 36 and such discharges were abolished by an injection of lidocaine into the tissue around ST 36. There was no change in the nerve discharges when applying the same acupuncture at either SP 6 or ST 25. Conelusion Manual acupuncture at ST 36 can evoke specific electrical discharge from the nerve innervated ST 36.

关 键 词：针刺 穴位 外周神经放电 大鼠 坐骨神经分支 手捻针 

分 类 号：R2-031[医药卫生—中西医结合]