再生障碍性贫血患者血清抑制CFU-MK的增殖及调控  

The proliferation and regulation of CFU-MK were inhibited by sera from patients with aplastic anemia

作　　者：宋国齐[1] 刘红[1] 姜胜华[1] 丁润生[1] 

机构地区：[1]南通大学附属医院血液内科,南通226001

出　　处：《陕西医学杂志》2008年第3期278-279,共2页Shaanxi Medical Journal

基　　金：江苏省"135工程"医学重点人才基金资助项目(RC2002039)

摘　　要：目的:探讨再生障碍性贫血(再障)患者血小板减少的原因,建立模型并寻找治疗对策。方法:应用甲基纤维素半固体体外培养法建立CFU-MK培养体系;混合再障血清进行培养建立再障患者血小板减少的模型;使用不同的造血细胞生长因子进行组合观察模型中CFU-MK集落生成情况和集落中巨核祖细胞的含量。结果:经过14d的培养,模型组CFU-MK集落、集簇数量明显低于正常对照组,以TPO/IL-3/IL-11/FL细胞因子组合使模型组CFU-MK的数量恢复及巨核细胞的扩增效果最好。结论:本实验建立了CFU-MK体外培养体系及再障血小板减少的模型,体外试验表明治疗再障血小板减少以TPO/IL-3/IL-11/FL为最佳组合。Objects： To investigate the reasons and establish the model of thrombopenia of aplastic anemia, and explore the treatment strategy of thrombopenia of aplastic anemia. Methods： CFU-MK and the model of thrombopenia of aplastie anemia were analysed by methylcellulose culture or with sera from patients with apIastic anemia. The number of rnegakaryocyte colony-forming unit and the content of CD41 ＋ cells in CFU-MK were measured simultaneously. Results： After 14 clays of culture, the number of CFU-MK and megakaryocyte cluster-forming unit of the model groups were significantly less than that of the normal control groups. The combination of TPO/IL-3/IL-11/FL was the most suitable for the growth of CFU-MK and the expansion of megakaryoeytic progenitors incultured with sera from patientswith aplastic anemia. Conclusions ： It is concluded that the study has established the model of thrombopenia of aplastie anemia and the methods of culture of CFU-MK, The combination the TPO/IL-3/IL-11/FL was the best optional for the treatment of the thrombopenia of aplastie anemia in vitro.

关 键 词：贫血 再生障碍性血小板减少/血液 巨核细胞/血液 模型 生物学 

分 类 号：R752.120.5[医药卫生—皮肤病学与性病学] R644.03[医药卫生—临床医学]