当归芍药散对快速老化模型小鼠海马蛋白表达的影响  

Effect of powder of Chinese Angelica and Peony on expressions of hippocampal proteins in senescence accelerated mice

作　　者：耿淼[1] 蒋宁[1] 周文霞[1] 张永祥[1] 

机构地区：[1]军事医学科学院毒物药物研究所中药和神经免疫药理研究室,北京100850

出　　处：《第三军医大学学报》2008年第6期477-480,共4页Journal of Third Military Medical University

基　　金：国家重点基础研究发展规划项目("973"项目)(2004CB518907);国家科学自然基金(90709012;30772562)~~

摘　　要：目的研究当归芍药散(powder of Chinese Angelica and Peony,DSS)对快速老化模型小鼠(senescence-accel-erated mouse,SAM)海马蛋白表达的影响。方法选择12月龄SAM的快速老化亚系SAMP8和抗快速老化亚系SAMR1作为研究对象,分为SAMR1组、SAMP8组和SAMP8+DSS组(3.2g/kg),每组10只,连续给药30d。应用双向凝胶电泳结合质谱鉴定,观察DSS对SAMP8海马蛋白表达的影响。结果与SAMR1组比较,SAMP8组海马中有9个蛋白表达下调,13个蛋白表达上调;SAMP8+DSS组与SAMP8组比较,海马中有14个蛋白表达下调,21个蛋白表达上调。经肽质量指纹谱分析及蛋白数据库检索,表达有明显变化的蛋白根据功能可分为5类:线粒体相关蛋白、能量代谢相关蛋白、突触形成相关蛋白、细胞骨架蛋白、其他蛋白。结论SAMP8和SAMR1海马蛋白表达存在明显差异,DSS可影响快速老化模型小鼠海马蛋白的表达,其作用是多途径多靶点的。Objective To study the effect of powder of Chinese Angelica and Peony （DSS） on hippocampus proteome of senescence accelerated mice （SAM）. Methods used in our study, including 10 SAM-resistance/1 （SAMR1） and Thirty female mice aged 12 months were 20 SAM-prone/8 （SAMP8）. The difference of hippocampus proteome among SAMP8, SAMR1, SAMP8 treated with DSS intragastrically at dose of 3.2 g/kg once a day for 30 d （SAMP8 ＋ DSS）, was analyzed by two dimensional polyacryalmide gel electrophoresis （2DE）. Results Compared with SAMR1, the expressions of 13 proteins in hippocampus of SAMP8 were upregulated and that of 9 proteins were down-regulated significantly. Compared with SAMP8, the expressions of 21 proteins in hippocampus of SAMP8 ＋ DSS were up-regulated and that of 14 proteins were down-regulated siguifi cantly. Using MALDI-TOF-MS, the proteins with significant changes in expressions were identified by peptide fingerprinting map and the results were searched in MASCOT database, and sorted into 5 function groups including mitochondria-associated proteins, energy metabolism proteins, synaptic plasticity formation proteins, cytoskeletal proteins and others proteins. Conclusion The effects of DSS on hippocampus proteome of senescence accelerated mice were probably exerted via multi-target and multi-pathway mechanism.

关 键 词：当归芍药散 快速老化模型小鼠 海马 蛋白质组学 

分 类 号：R285.5[医药卫生—中药学] R289[医药卫生—中医学]