黄瓜果实扩张蛋白基因克隆与分析(英文)  

Clone and Analysis of Expansin Gene of Cucumber Fruit

作　　者：孙涌栋[1] 张兴国[2] 罗未蓉[1] 李新峥[1] 刘遵春[1] 

机构地区：[1]河南科技学院园林学院,河南新乡453003 [2]西南大学园艺园林学院,重庆400715

出　　处：《西北植物学报》2008年第2期233-236,共4页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家“863”计划项目(2003AA207120);教育部优秀青年教师资助计划项目[教育司(2003)355号]资助;河南科技学院重点科研项目资助基金(6011)

摘　　要：以非单性结实的全雌黄瓜为实验材料,利用cDNA-AFLP技术比较了授粉前后黄瓜幼果的基因表达差异。以ASE-AT/TAQ-CAG为引物对从授粉后黄瓜幼果组织中分离到1条特异片段,该片段仅在授粉后黄瓜幼果组织中表达,将该片段回收测序并翻译成氨基酸序列,用blastp程序在NCBI GenBank数据库中进行同源性检索和相似性比对,结果发现该片段推导的氨基酸序列与黄瓜扩张蛋白CsEXP1～9的相似性依次分别为71%、58%、63%、75%、85%、82%、67%、68%和85%,可能是一个新的黄瓜扩张蛋白基因,命名为CsEXP10,表明扩张蛋白基因可能与黄瓜果实膨大生长有密切关系。Using full-female cucumber without non-parthenocarpy as material,cDNA-AFLP technique was employed to analyzed the differential expression of genes in young fruit of cucumber（Cucumis sativus L.） before/after pollination.A fragment only expressed in young fruit of cucumber after pollination was obtained via selective amplification with the primer pair ASE-AT/TAQ-CAG.After recollection,sequencing and translation,the results of blastp in GenBank of NCBI indicated that the deduced amino acid sequences of the fragment shared the highest similarity with CsEXP1～9 and the similarity were 71%,58%,63%,75%,85%,82%,67%,68% and 85%,respectively.So it was a new gene encoding expansin and was named as CsEXP10,which suggested that expansin might be highly correlated with the expansive growth of cucumber fruit.

关 键 词：黄瓜(Cucumis SATIVUS L.) 果实膨大生长 CDNA-AFLP 扩张蛋白 

分 类 号：Q789[生物学—分子生物学]