接苯工人胸腺近期输出功能和外周血T细胞亚群变化  

Changes of Recent Thymic Output Function and T Lymphocyte Subgroup in Peripheral Blood of Benzene Exposed Workers

作　　者：李萡[1] 李扬秋[1] 杨力建[1] 陈少华[1] 刘薇薇[2] 陈嘉榆[2] 

机构地区：[1]暨南大学医学院血液病研究所,广东广州510632 [2]广州市第十二人民医院,广东广州510620

出　　处：《工业卫生与职业病》2008年第2期68-71,共4页Industrial Health and Occupational Diseases

基　　金：国家教育部科技重点项目〔教技司(2001)235号01091〕;广州市科委科技攻关项目(2001-Z-037-01-3);血液病学国务院侨办重点学科基金

摘　　要：目的分析接苯工人外周血单个核细胞中T细胞受体删除DNA环(TRECs)和T淋巴细胞亚群的含量,了解接苯后工人胸腺近期输出功能和外周T淋巴细胞亚群变化,为接苯后机体细胞免疫改变提供资料。方法利用间接免疫荧光法检测健康人和接苯工人外周血单个核细胞中CD3+,CD4+,CD8+的含量;采用实时荧光定量聚合酶链反应(real time PCR)和TaqMan方法检测标本外周血单个核细胞中TRECs的含量。结果在接苯工人外周血白细胞总数正常情况下,接苯组CD8+含量显著增加,CD4+/CD8+比值为(1.13±0.78),低于健康人(1.43±0.25)(P<0.05)。每1000个外周血单个核细胞中接苯组与对照组TRECs含量分别为(3.90±3.58)和(7.39±6.83),两者相比,差异有统计学意义(P<0.05)。结论长期低剂量接苯可能引起一些工人的胸腺近期输出功能低下,导致机体细胞免疫功能障碍。Objective Through analyzing the ratio of T lymphocyte subgroup and the content of T cell receptor excision DNA circles （TRECs） within peripheral blood mononuclear cells （PBMCs） to evaluate the recent thymic output function and the distribution of T cell subgroup in benzene exposed workers so as to present data for study on the impact of benzene exposure on T cell immune function. Methods T lymphocyte subgroups were assessed by indirect fluoroimmunoassay and quantitative detection of TRECs in DNA of PBMCs was preformed by real time PCR using TaqMan technique in benzene exposed workers and normal individuals. Results The CD8^＋ significantly increased and the CD4 ＋/CD8^＋ decreased in benzene exposed workers（P〈0. 05）. The levels of TRECs were （3.90 ± 3.58） /1000 PBMCs and （7. 39 ±6.83） /1000 PBMCs in the control and benzene exposed groups. The level of TRECs in benzene exposed groups decreased significantly compared with that of the control groups（P〈0. 05）. Conclusions The results indicate that longterm low dose benzene exposure might induce the recent thymic output function to decrease in benzene exposed workers causing the cellular immunity disorder.

关 键 词：苯 胸腺输出功能 实时荧光定量聚合酶链反应 T淋巴细胞亚群 T细胞受体删除DNA环 

分 类 号：R135.12[医药卫生—劳动卫生]