乳腺癌腋窝淋巴结免疫功能的探讨  

Distribution on immune function of the axilla lymph nodes in breast cancer.

作　　者：陆云飞[1] 张小彬[1] 

机构地区：[1]广西医科大学第一附属医院胃肠腺体外科,广西南宁530021

出　　处：《中国实用外科杂志》2008年第3期211-212,共2页Chinese Journal of Practical Surgery

基　　金：广西科技攻关项目课题(No0322025-1)

摘　　要：目的研究乳腺癌腋窝淋巴结发生癌转移和未发生癌转移时的免疫功能。方法2004年8月至2005年7月采用流式细胞技术检测乳腺癌病人乳腺癌前哨淋巴结(SLN)与乳腺癌非前哨淋巴结(NSLN)中免疫细胞CD3+T、CD4+T、CD8+T、CD16+NK、CD19+B的比例,并相互比较。结果淋巴结未发生癌转移时,SLN与NSLN的免疫细胞CD3+T、CD4+T、CD8+T、CD16+NK、CD19+B的比例差异无统计学意义(P>0.05);而且当SLN与NSLN发生癌转移后,它们的免疫细胞的比例也无差异。但SLN发生癌转移与未发生癌转移时相比,其CD4+T、CD8+T、CD16+NK的比例发生显著改变(P<0.05),CD3+T、CD19+B的比例改变无统计学意义(P>0.05)。结论当腋窝淋巴结未发生癌转移时,它仍有正常的免疫功能。当腋窝淋巴结发生癌转移后,它的免疫微环境发生了改变,免疫功能呈现一种抑制状态。Objective To investigate the immune function of Axilla lymph nodes which cancer cells metastasis and not metastasis in breast cancer. Methods It used flow cytometry to calculate the percent of lymphocyte subsets of the axilla lymph nodes and compare the result with each other between August 2004 and July 2005. Results Whether cancer cells metastasis to the axilla lymph nodes or not ,the percentage of lymphocytes CD3^＋ T ,CD4^＋ T ,CD8^＋ T ,CD16^＋ NK,CD^19 ＋ B had no statistical difference （ P 〉0. 05）. When cancer cells metastasis to SLN,the percentage of lymphocytes CD4^ ＋T,CD8^＋T, CD16^＋ NK had statistical difference （ P 〈0. 05 ） ,and the percentage of CD3^＋T,CD19^＋ B had no statistical difference （ P 〉 0. 05）. Conclusion When the axilla lymph nodes has no cancer cells metastasis, it ＇ s immune function is natural. Once lymph nodes has cancer cells metastasis,it＇s immune entironment is transformation,and so the immune function is bated.

关 键 词：乳腺癌 免疫细胞 免疫功能 流式细胞技术 前哨淋巴结 

分 类 号：R6[医药卫生—外科学]