致敏小鼠树突状细胞和T细胞PD-1配体/受体的表达  

Expressions of PD-1 and its ligands in spleen DCs and T cells of allergic mice

作　　者：曾雪峰[1] 宋云熙[1] 夏俊波[1] 王长征[1] 

机构地区：[1]第三军医大学新桥医院全军呼吸内科研究所,400037

出　　处：《免疫学杂志》2008年第2期123-126,共4页Immunological Journal

基　　金：国家自然科学基金资助项目(30640094)

摘　　要：目的探讨致敏小鼠PD-1信号通路是否有改变。方法RT-PCR法分别检测致敏及正常小鼠树突状细胞PD-1配体PD-L1、PD-L2及T细胞PD-1受体的mRNA表达情况,流式细胞检测PD-1和配体PD-L1、PD-L2的表达。结果①致敏小鼠树突状细胞PD-L1和PD-L2的mRNA表达均显著低于正常小鼠,致敏小鼠T细胞PD-1的mRNA表达也显著低于正常小鼠(P<0.05)。②流式细胞检测致敏小鼠树突状细胞PD-L1、PD-L2表达和T细胞PD-1的表达也显著低于正常小鼠。结论致敏小鼠PD-1信号表达缺陷。Objective To investigate the expression of PD- 1 in allergic mice. Methods The mRNA levels of PD- 1 ligands and receptors in spleen DC and T cells of allergic mice and normal mice were measured by RT-PCR. The expression of PD-L1 and PD-L2 in spleen DC and the expression of PD-1 in T cells were detected by flow cytometry. Results OThe mRNA levels of PD-L1 and PD-L2 in DC and the mRNA levels of PD-1 in T cells were significantly decreased in allergic mice （ P 〈 0.05）. （1）Expressions of PD-L1, PD-L2, and PD-1 in spleen cells were significantly decreased in allergic mice. Conclusion The expression of PD-1 is decreased in allergic mice.

关 键 词：PD-1 变应性 免疫耐受 

分 类 号：R392[医药卫生—免疫学]