结直肠癌患者血清Syk基因甲基化与术后复发转移和预后的关系  

Correlation of hypermethylation of Syk gene in the serum with postoperative recurrence, metastasis and survival in colorectal cancer patients

作　　者：杨祖立[1] 康亮[1] 王磊[1] 汪建平[1] 彭俊生[1] 黄美近[1] 兰平[1] 黄奕华[2] 何裕隆[2] 

机构地区：[1]中山大学附属第六医院(附属胃肠肛门医院)胃肠外科,广州510655 [2]中山大学附属第一医院胃肠外科

出　　处：《中华普通外科杂志》2008年第1期36-39,共4页Chinese Journal of General Surgery

基　　金：教育部博士点基金资助（20020558050）;广东省自然科学基金博士启动项目（06300853）

摘　　要：目的探讨血清Syk基因甲基化与结直肠癌术后复发转移和预后之间的关系。方法收集120例结直肠癌患者的组织和血清标本，采用甲基化特异性PCR（MSP）检测Syk基因的甲基化状态。结果120例结直肠癌患者中37例肿瘤组织发生Syk基因甲基化，其相应血清中32例可检测到同样的甲基化模式；而肿瘤组织未发生甲基化的83例患者的血清中均未检测到甲基化的Syk基因。血清Syk基因甲基化组的淋巴结转移率明显高于无甲基化组（68．8％比38．6％，x^2=8．55，P=0．003）；前者的术后转移复发率也明显高于后者（34．4％比9．1％，x^2=10．46，P=0．001）；血清Syk基因甲基化组的3年生存率明显低于无甲基化组（65．1％比90．6％，x^2=7．818，P=0．006）。结论血清Syk基因甲基化状态与肿瘤组织的甲基化状态密切相关，可以作为结直肠癌患者淋巴结转移、预测复发和判断预后的一个指标。Objective To investigate the correlation of methylation status of Syk gene in serum with postoperative recurrence, metastasis and survival in colorectal cancer patients. Methods MSP technique was used to analyze the methylation status of Syk gene in both cancer tissue and corresponding serum of 120 CRC patients. Results （1）of 120 patients, 37 patients were found with Syk hypermethylation in tumor tissues, among which 32 cases with same methylation status of Syk gene in the serum. There was no aberrant methylation of Syk gene both in the serum and cancerous tissues in 83 cases; （2） Lymph node metastasis was found in 68. 8% cases in the group with methylated Syk in serum, significantly higher than that of nonmethylation group （38. 6%, P = 0. 003 ）. The difference of postoperative tumor recurrence rate was also significant between the two groups （34. 4% vs. 9. 1%, P =0. 001 ） , so as to the 3-year survival rate （65. 1% vs. 90. 6%, P =0. 006）. Conclusions Hypermethylation status of Syk gene in serum was closely correlated with that in tumor tissues, which can predict lymph node status, postoperative tumor recurrence and postoperative 3-year survival.

关 键 词：结直肠肿瘤 甲基化 肿瘤转移 预后 

分 类 号：R686[医药卫生—骨科学]