TGF-β1修饰的未成熟树突状细胞对大鼠移植小肠细胞凋亡的影响  

Effects of imDC transfected by TGF-β1-recombinated adenovirus vector on apoptosis of grafts in rat small bowel transplantation

作　　者：李纪鹏[1] 黄怡[1] 王为忠[1] 董光龙[1] 杜建军[1] 陈冬利[1] 季刚[1] 刘骥[1] 

机构地区：[1]中国人民解放军第四军医大学西京医院胃肠外科,西安710032

出　　处：《中华普通外科杂志》2008年第2期94-97,共4页Chinese Journal of General Surgery

基　　金：国家自然科学基金资助项目（30571803）

摘　　要：目的探讨经TGF-β1修饰的未成熟树突状细胞（imDC）预处理大鼠小肠移植受体后移植肠细胞凋亡的变化及意义。方法选用近交系F344／N和BN大鼠建立全小肠异位移植模型，分4组，每组24只。A组：同基因移植组（BN→BN）；B组：异基因移植组（F344／N→BN）；C组：F344／N→BN异基因移植＋TGF-β1基因转染imDC；D组iF344／N→BN异基因移植＋TGF-β1基因转染imDC＋FK506。术后3、5、7d各处死6只，取出移植肠。行免疫组化检测Bcl-2和Bax表达，TUNEL及电镜观察细胞凋亡。同期进行移植肠组织病理学检查。结果C组中Bcl-2在术后有轻度下降，而Bax的表达则略有升高，但明显低于B组，差异有统计学意义（P〈0．05）；D组术后Bcl-2及Bax的表达与A组无明显差异。C组的凋亡细胞数在术后逐渐增加，但数量始终较少，与B组比较差异有统计学意义（P〈0．05）；D组仅见少量凋亡细胞。结论经TGF-β1基因转染的imDC预处理受体可以抑制细胞凋亡，从而减轻小肠移植术后急性排斥反应的程度。Objective To investigate effects of imDC transfected by TGF-β1-recombinated adenovirus vector on apoptosis of grafts in rat small bowel transplantation. Methods Heterotopic small bowel transplantations were performed. The recipients were divided into four groups（24 in each group） ： group A isograft （BN→BN）, group B allograft （F344/N→BN）, group C TGF-β1 treated F344/N→BN allograft group, and group D FK506 ＋ TGF-β1 treated F344/N→BN allograft group. On POD 3,5 and 7,the entire graft jejunum from 6 recipients of each group were taken for determination of Bcl-2 and Bax by immunochemistry, for apoptosis in grafts by TURNEL and for ultramicrostructure observation by transmission electron microscope respectively. Results In TGF-β1 allograft group, the levels of Bcl-2 decreased, while the levels of Bax elevated （ but it was significantly lower than that in group B, P 〈 0. 05 ）. Small portion of apoptosis could be observed. There were no significant differences in the expression of Bcl-2 and Bax between group D and group A. Cell apoptosis in group C increased gradually but still significantly lower than that in group B （ P 〈 0. 05）. In FK506 ＋ TGF-β1 allograft group, The levels of Bcl-2 and Bax did not change significantly when compared with that of isograft group （ P 〉 0. 05）, apoptosis could only be observed sporadically. Conclusion imDC transfected by TGF-β1-recombinated adenovirus vector alleviates acute rejection by inhibiting graft apoptosis in small bowel transplantation.

关 键 词：受体 转化生长因子β 树突细胞 小肠移植 细胞凋亡 

分 类 号：R686[医药卫生—骨科学]