重离子与X射线辐射人肝癌细胞后细胞凋亡及STAT-3基因表达的研究  

Apoptosis and STAT-3 expression of human hepatoma SMMC-7721 cells following X-ray and heavyion irradiations

作　　者：马建勋[1] 叶兰萍[1] 杨天照[1] 李荣范[1] 司若煌[1] 

机构地区：[1]甘肃省人民医院普外科,兰州730000

出　　处：《中华普通外科杂志》2008年第2期101-103,共3页Chinese Journal of General Surgery

摘　　要：目的探讨重离子辐射对人肝癌细胞系SMMC-7721细胞增殖及细胞凋亡的影响，以及STAT-3基因表达的变化。方法采用克隆存活及流式细胞技术，探讨体外培养的人肝癌细胞系SMMC-7721经X射线及重离子辐射后，细胞周期及细胞凋亡的变化，并采用RT—PCR法及Western blotting法检测肝癌细胞系SMMC-7721 STAT-3基因的表达。结果人肝癌细胞系SMMC-7721经不同剂量重离子辐射后，随辐射剂量增加，细胞存活率（SF）明显降低（分别t=0．89、0．76、0．41、0．23，均P〈0．05）。流式细胞仪检测显示细胞周期G2／M期阻滞增强，重离子较X射线作用更明显（分别t=1．31、8．26，P〈0．05），重离子辐射较X射线对细胞凋亡率影响更大（x^2=48．46，P〈0．01）。Western blotting和RT-PCR法均显示随着辐射剂量的增加STAT-3基因的表达逐渐增强。结论重离子较X射线可更明显地诱导癌细胞凋亡，降低细胞存活率，人肝癌细胞系SMMC-7721经X射线及重离子辐射后，细胞凋亡与STAT-3基因的表达相关。Objective To study the effect of X-ray and heavy-ion irradiations on cell growth and apoptosis of human hepatocellular cell line SMMC-7721 and the role of stat-3 expression in the induction of cell apoptosis. Methods Flow cytometry （FCM） was used to determine cell circle and apoptosis in cultured SMMC-7721 after X-ray or heavy-ion irradiation respectively. Stat-3 protein and mRNA expressions were measured with Western blotting analysis and reverse transcription polymerase chain reaction （RT-PCR）. Results The viability of SMMC-7721 cells decreased （ vs. control, P 〈 0. 05） along with the dose increase of carbon ion beam. FCM showed more SMMC-7721 cells arrested at G2/M after heavy-ion irradiation compared with X-ray irradiation （ t = 1. 31, t = 8.26, P 〈 0. 05 ）. Also, heavy-ion irradiation induced higher apoptosis rate of SMMC-7721 cell lines when compared with X-ray irradiation （ x^2 = 48.46, P 〈0. 01 ）. Carbon ion irradiation significantly promoted the expression of stat-3 gene compared with the X-ray irradiation as measured by Western blotting and RT-PCR. Conclusion Heavy ion beam induces cell apoptosis and decreases the viability of SMMC cell lines more effectively than X-ray. The apoptosis rate is positively correlated with the transcription of stat-3 in human hepatoma SMMC-7721 cells after exposure to X-ray and heavy ion beam.

关 键 词：癌 肝细胞 重离子 细胞凋亡 细胞系 信号转导激活蛋白3 

分 类 号：R686[医药卫生—骨科学]