不同环境条件下稻米组氨酸和精氨酸的胚乳和母体植株QTL分析  

Mapping and Analysis of QTLs on Maternal and Endosperm Genomes for Histidine and Arginine in Rice (Oryza sativa L.) across Environments

作　　者：郑希[1] 吴建国[1] 楼向阳 徐海明[1] 石春海[1] 

机构地区：[1]浙江大学农学系,浙江杭州310029 [2]Department of Psychiatry and Neurobehavioral Sciences, University of Virginia, Charlottesville,VA 22911, USA

出　　处：《作物学报》2008年第3期369-375,共7页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(30571198);国家高技术研究发展计划(863计划)项目(2006AA100101);国家重点基础研究发展计划(973计划)项目(2007CB109000);浙江省重大科技攻关项目(011102471);浙江省151人才基金项目

摘　　要：利用汕优63重组自交系与双亲回交产生的BC1F1和BC2F1群体,采用新发展的包括环境互作效应在内的多遗传体系QTL作图方法和基因定位软件,对稻米两种半必需氨基酸(组氨酸和精氨酸)进行三倍体胚乳和二倍体母体植株等不同遗传体系的QTL定位分析。共检测到10个控制组氨酸含量的QTL以及8个控制精氨酸含量的QTL。全部QTL均具有极显著的三倍体胚乳和二倍体母体植株基因的加性主效应,其中4个QTL具有显著或极显著的三倍体胚乳显性主效应,7个QTL还具有明显的环境互作效应。As a staple cereal crop in the world, rice feeds more than 50% of the world population. The improvement for rice quality including protein content and kinds of amino acid contents is an important work to meet the demands of a growing population. The genetic analysis on rice indicate that the inheritance of nutrient quality traits is complex, which involve the genetic effects from the triploid endosperm nuclear genes and the diploid maternal plant nuclear genes, being further partitioned into additive and dominance effects, and their genotype × environment （GE） interaction effects. So far, the magnitude and prevalence of interactions between quantitative trait loci （QTL） of the triploid endosperm genome or diploid maternal genome for rice traits are still largely unknown. Therefore, it is necessary to study the genetic main effects and GE interaction effects of the QTL from different genomes across environments. Investigations to identify QTL governing histidine （His） and arginine （Arg） contents of rice were conducted using the newly developed QTL mapping method including endosperm and maternal main effects and their GE interaction effects on quantitative traits of seed in cereal crops. Two backcross populations, which were a set of 241 RILs derived from an elite hybrid cross of ‘Shanyou 63’ crossed with ‘Zhenshan 97’ （BC1F1） or ‘Minghui 63’ （BC2F1）, were used in two environments. The results showed that significant differences were found between the two parents for both quantitative quality traits. His and Arg of rice for Zhenshan 97 were higher than those for Minghui 63. The distributions of phenotypic values for His and Arg in BC1F1 （RILs × P1） and BC2F1 （RILs × P2） populations revealed normal distributions approximately. Both backcross populations also showed varying distributions in 1999 and 2000, implying that both amino acid traits were subjected to the modification by environments. A total of ten QTL associated with His content were mapped on chromosomes 1, 2,

关 键 词：水稻(Oryza SATIVA L.) QTL定位 组氨酸 精氨酸 胚乳 母体植株 环境互作效应 

分 类 号：S511[农业科学—作物学]