柱花草子叶组织培养多因素组合数学模型的研究  

Mathematical Model of Multifactor Combination on Tissue Culture for Stylosanthes Cotyledon

作　　者：钟军[1] 郑卓[2] 智旭丹[3] 

机构地区：[1]湖南农业大学农学院,湖南长沙410128 [2]井冈山大学生命科学学院,江西吉安343009 [3]湖南农业大学细胞工程实验室,湖南长沙410128

出　　处：《作物学报》2008年第3期534-538,共5页Acta Agronomica Sinica

基　　金：湖南省湖南农业大学人才引进资助项目(2003YJ007)

摘　　要：采用正交试验研究了影响柱花草子叶组织培养的可控因子,并建立了柱花草子叶培养对可控因子反应的数学模型。结果表明,可控因子与柱花草愈伤组织诱导率、芽分化系数和生根率之间存在真实的回归关系,最佳回归模型分别为Y=86.90+3.34X1+1.36X3+2.86X1X3、Y=7.70+1.52X2和Y=299.30+6.39X1+4.32X1X2。从模型的解析可看出,对愈伤组织诱导率影响最大的是NAA,其次是NAA与蔗糖之间的互作,最后是蔗糖;对芽分化有影响的是KT;对生根率影响最大的是NAA,其次是NAA/IAA的比值。Stylosanthes guianensis is regarded as one of the newest green leguminous forage in tropical and subtropical regions of China.It is a species of stylosanthes（Stylosanthes spp.）,which has good traits including high dry matter yield,cold tolerance,anthracnose resistance and late maturity.But there were few reports on optimal media recipes for tissue culture in Stylosanthes guianensis.To investigate the main factors affecting tissue culture,callus was induced with a cultivar Reyan 10（Stylosanthes guianensis cv.）,surviving callus was cultured on different media after 2 weeks,then,the buds with 2-3 cm in length were regenerated on root induction medium after 3 weeks,finally they were transplanted into soil as the roots had formed after 2-3 weeks.Meanwhile the main medium components affecting the tissue culture were determined with orthogonal design,and mathematical model between the medium components and traits was established.The results was showed that there was a real regression relation between regulated factors and rate of callus induction,coefficient of bud differentiation,rate of root induction,with the equations of Y = 86.90＋3.34X1＋1.36X3＋2.86X1X3,Y=7.70＋1.52X2,and Y=299.30 ＋6.39X1＋4.32X1X2,respectively.For callus induction,NAA was the most efficient factor,interaction between NAA and sucrose was the second factor,and sucrose was the last one.Also,the important factors were KT for bud induction as well as NAA and NAA/IAA for root induction.

关 键 词：柱花草 子叶培养 多因素组合 数学模型 正交设计 

分 类 号：S541.9[农业科学—作物学]