低频电针足三里对酒依赖大鼠饮酒行为的影响  

Effects of Low Frequency Electroacupuncture at Zu San-Li on Ethanol Taking Behavior in Ethanol Dependent Rats

作　　者：王育红[1] 俞建良[2] 黄劲松[1] 郝伟[1] 张富强 周文华 谌红献[1] 周旭辉[1] 王绪轶[1] 刘义军[2] 刘惠芬 唐甩恩 杨国栋 

机构地区：[1]中南大学湘雅二医院精神卫生研究所,长沙410011 [2]上海中医药大学,上海200032 [3]宁波戒毒研究中心暨宁波大学医学院行为神经科学研究中心,宁波315010

出　　处：《中国心理卫生杂志》2008年第3期157-163,共7页Chinese Mental Health Journal

基　　金：973项目(CB2003515403、CB2003515404);国家自然科学基金(30370522、30500180、30670676)

摘　　要：目的:研究低频电针(2Hz)足三里对酒依赖大鼠饮酒行为的影响。方法:共24只SD大鼠(酒依赖模型组18只,对照组6只)。采用6%(v/w)的酒精水溶液作为SD大鼠唯一的饮水来源共30天建立酒精躯体依赖模型,进一步将其分为戒断组、捆绑组、电针组各6只大鼠。分别对酒精依赖大鼠进行急性(电针一次)和慢性(电针六次)低频电针足三里处理,观察急慢性电针对大鼠饮酒量和酒精偏爱程度(酒精/水自由选择)的影响。结果:急性电针足三里处理大鼠饮酒量和酒精偏爱组间比较差异有显著性(F(2,15)=7.034,P=0.007;F(2,15)=4.810,P=0.024),电针组饮酒量和酒精偏爱较对照组(捆绑对照和非捆绑对照)均降低,其中三组饮酒量和酒精偏爱均值和标准差分别为(4.2±0.4,9.6±0.9,8.0±0.7)及(4.3±0.2,7.5±0.7,7.8±0.7);差异有统计学意义(P<0.001)。慢性电针足三里处理后大鼠饮酒量和酒精偏爱程度与对照组(捆绑对照和非捆绑对照)比较均无显著性差异。结论:急性低频电针足三里能抑制酒精依赖大鼠的饮酒行为。Objective：To evaluate the effects of low frequency electroacupuncture（EA）at Zu San-Li on ethanol taking behavior in ethanol dependent rats.Methods：18 Rats were made ethanol-dependent by supplying ethanol solution（6%,v/w）as the sole drinking source for 30 days.Total ethanol intake and ethanol（6%）/water choice preference were measured after acute（one time）or chronic（six times）EA at Zu San-Li.Two control groups were set as constrained control,non-constrained control.Results：There were significant differences in both total amount of ethanol intake and ethanol preference（ethanol/water free choice）among three groups after acute EA at Zu San-Li（F（2,15）=7.034,P =0.007;F（2,15）=4.810,P=0.024）.The rats received acute EZ had lower ethanol intake and ethanol preference than the two control groups（4.2±0.4,9.6±0.9,8.0±0.7 and 4.3±0.2,7.5±0.7,7.8±0.7;P〈0.001）,but this effects were not observed after chronic EA treatment.Conclusion：Acute low frequency EA at Zu San-Li could attenuate ethanol intake and preference in ethanol-dependent rats.

关 键 词：成瘾行为 酒依赖 大鼠 电针 足三里穴 饮酒行为 动物试验 

分 类 号：Q42[生物学—神经生物学] R595.6[生物学—生理学]