检测心肌细胞钾离子通道蛋白活化水平方法的优化  

Optimization of Method for Detecting the Activation of Potassium Channels in Rat Cardiac Muscle Cells

作　　者：王曦[1] 张磊[1] 周士胜[2] 邹伟[1] 

机构地区：[1]辽宁师范大学生命科学学院,大连116029 [2]大连大学医学部,大连116622

出　　处：《生物工程学报》2008年第3期521-524,共4页Chinese Journal of Biotechnology

基　　金：国家自然科学基金资助(No.30570665)~~

摘　　要：介绍了一种如何合理的利用蛋白质免疫沉淀和蛋白质免疫印迹相结合的方法检测大鼠心肌细胞钾离子通道蛋白Kv1.2和Kv1.5的表达与活化水平。实验结果表明,与单独利用免疫印迹的方法相比较,本实验是对钾离子通道蛋白及其它亚家族的钾通道蛋白磷酸化表达水平检测方法的一种优化,从而获得一套可行、简单、合理的实验方案,同时也提高了检测的准确性,敏感性及特异性。To detect the phosphorylation of potassium channel protein （Kv1.2 and Kv1.5） in rat cardiac muscle cells accurately, we applied the combined method of immunoprecipitation and Western blot in this study. Compared with using Western blot alone, the combination of immunoprecipitation and Western blot displayed high sensitivity to detect the activation of potassium channel pro- teins. Because of its simplicity, quickness and reproducibility, we find that this method was promising for detecting the phosphorylation of Kv1.2 and Kv1.5 proteins or other potassium channel proteins in rat cardiac muscle cells.

关 键 词：蛋白质免疫沉淀 大鼠心肌细胞 钾离子通道蛋白 

分 类 号：Q51-3[生物学—生物化学]