乌司他丁对内毒素诱导大鼠急性肾损伤作用的研究  

Ulinastatin provides protection against endotoxin-induced acute kidney injury in rats

作　　者：李原媛[1] 刘虹[1] 李芳芳[1] 王波[2] 

机构地区：[1]山西医科大学第一医院重症医学科,太原030001 [2]武警山西总队医院外一科

出　　处：《中国药物与临床》2008年第3期200-202,共3页Chinese Remedies & Clinics

摘　　要：目的探讨乌司他丁(UTI)在内毒素(LPS)诱导的大鼠急性肾损伤(AKI)发病中的影响。方法健康雄性Wistar大鼠30只随机分为3组:健康对照组:经尾静脉注射1ml生理盐水;LPS组:经尾静脉注射LPS(5mg/kg体质量),复制AKI模型;LPS+UTI组:在按与LPS组相同标准给LPS的同时给予UTI10万U/kg体质量。观察6h,酶联免疫吸附法测血清及尿液中细胞因子白细胞介素(IL)-18的含量,苦味酸法检测血肌酐(SCr)值并观察各组大鼠电镜下肾小管的形态改变。结果LPS组大鼠SCr较正常对照组及LPS+UTI组显著升高,LPS组血清及尿液中IL-18的水平也明显高于健康对照组及LPS+UTI组。电镜下观察健康对照组大鼠肾小管上皮细胞核大而圆,刷状缘及线粒体完整;LPS组可见肾小管上皮细胞核固缩,线粒体嵴断裂,空泡变性,微绒毛连续性破坏;而LPS+UTI组则基本接近正常的肾小管上皮细胞的形态,仅见到少量的线粒体嵴溶解。结论UTI可降低炎性细胞因子IL-18的表达水平,对LPS诱导大鼠AKI时肾脏具有保护作用。Objective To explore the role of Ulinastatin （UTI） in lipopolysaccharide （LPS）-induced acute kidney injury （AKI） in rats. Methods Thirty male Wistar rats were randomized to receive caudal injection of 1 ml normal saline （control group）, or 5 mg/kg LPS （LPS group） or 5 mg/kg LPS plus 105 U/kg UTI （LPS＋UTI group）. Six hours later, the blood and urine levels of IL-18 were determined with enzyme-linked immunosorbent assay （ELISA）, serum creatinine （SCr） with Jaffe＇s reaction and the morphology of kidneys observed under an electron microscope. Results The level of Scr was significantly elevated in the LPS group compared with normal controls and the LPS＋UTI group, as were levels of blood and urine IL-18. Microscopically, the renal tubular epithelium in normal rats showed large and round-shaped nuclei, intact brush border and mitochondria, whereas pyknosis, rupture of mitochondrial cristae, vacuolization and disrupted microvilla were found for the LPS group in contrast to the nearly normal morphology in the LPS＋UIT group except for sparsely identifiable resolution of mitochondrial cristae. Conclusion UTI may reduce the expression of pro-inflammatory IL-18, hence its protection against LPS-indueed AKI in rats.

关 键 词：肾疾病 白细胞介素18 乌司他丁 

分 类 号：R96[医药卫生—药理学]