参附注射液对急性坏死性胰腺炎大鼠肠黏膜屏障保护作用的研究  

Effects of Shenfu on the damage of intestinal mucosa with acute necrotizing pancreatitis in rats

作　　者：胡占良[1] 吕志武[2] 张新宇[1] 姜明山[1] 韩德恩[1] 

机构地区：[1]哈尔滨医科大学附属第二医院肝胆胰及腹腔镜外科,哈尔滨150086 [2]哈尔滨医科大学附属第二医院消化内科,哈尔滨150086

出　　处：《中华胰腺病杂志》2008年第1期24-27,共4页Chinese Journal of Pancreatology

摘　　要：目的探讨参附注射液对ANP大鼠肠黏膜屏障的保护作用及机制。方法雄性Wister大鼠21只，按随机分组法分为对照组、ANP组及参附治疗组（SF组），检测各组血浆MDA、MPO、SOD、TNF—α及小肠组织MDA、NO、MPO含量；免疫组化方法检测小肠组织NF—κB、ICAM-1及iNOS的蛋白表达。结果ANP组血浆MPO、MDA、SOD、TNF—α量分别为（390．22±24．58）U／L、（41．79±5．68）nmol／L、（108．74±12．98）U／ml和（5．54±0．31）fmol／ml；肠组织MPO、MDA、NO含量分别为（270．96±31．86）U／g湿片、（7．61±2．02）nmol／g port和（51．21±46．98）μmol／g prot；NF—κB、ICAM-1及iNOS蛋白阳性表达细胞数分别为85．68±7．79、0．218±0．035和0．098±0．016。SF组相对应值分别为（279．68±50．19）U／L、（31．07±3．92）nmol／L、（123．45±7．94）U／L和（4．82±0．24）fmol／L；（214．46±19．64）U／g湿片、（2．88±1．48）nmol／g port和（20．27±7．92）μmol／g port；19．87±7．88、0．124±0．018和0．069±0．024。各项指标两组间差异均非常显著（P〈0．01）。结论参附注射液对ANP大鼠肠黏膜屏障有保护作用，其机制可能通过抑制NF—κB的表达，减少TNF—α、ICAM-1及iNOS的生成，从而改善肠道微循环，减轻肠组织炎症反应和减少氧自由基所致。Objective To investigate the role of Shenfu injection on intestinal mucosa of experimental acute necrotizing pancreatitis （ANP） in rats. Methods Twenty and one Wister rats were randomized into the control group, ANP group and Shenfu group. The serum level of MDA, MPO, SOD, TNF-α and the intestines concentrations of MDA, NO, MPO was measured. The NF-κB, ICAM-1, iNOS expression in the intestines were detected by immunohistochemistry. Results The values of serum MPO （279.68 ±50. 19 U/L）, MDA （31.07 ±3.92mmol/L）, SOD （ 123.45 ±7.94U/ml）, TNF-α（4.82±0.24 fmol/ml） and the intestines of MDA （2.88 ± 1.48 nmol/g port）, NO （20.27 ±7.92 μmol/g port）, MPO （214.46 ± 19.64 U/g） after establishment of the ANP model in Shenfu group were significantly lower than that in ANP group （ P 〈 0.01 ） but lower than that in the control group （P 〈 0. 01 ）. The values of expression of NF-κB, ICAM-1 and iNOS of intestines after establishment of the ANP model in Shenfu-group were significantly lower than that in ANP group （P 〈 0.01 ） but higher than that in the control group （P 〈 0.01 ）. Conclusions Shenfu injection can protect the early musocal injury of intestine of ANP, which may improve the intestinal microcirculation, restrain the production of NF-κB, reduce the production of TNF-α, ICAM-1 and iNOS, alleviate the inflammation of intestine, and decrease the injury of oxygen free radicals.

关 键 词：胰腺炎 急性坏死性 NF—κB 肿瘤坏死因子α 过氧化物酶 参附注射液 肠黏膜屏障 

分 类 号：R285.5[医药卫生—中药学] R259.416.1[医药卫生—中医学]