过氧化氢-乙醚法制备脱蛋白骨的理化性质研究  

The Physical and Chemical Character of Deproteinized Bone by Hydrogen Dioxide and Diethyl Ether

作　　者：曹凯[1] 舒勇[1] 韩智敏[1] 范伟[2] 安洪[2] 蒋电明[2] 段满生[1] 顾玉荣[3] 

机构地区：[1]南昌大学第一附属医院骨科,南昌330006 [2]重庆医科大学附属第一医院骨科,重庆400016 [3]南昌大学第二附属医院骨科,南昌330006

出　　处：《江西医学院学报》2008年第1期5-8,F0003,共5页Acta Academiae Medicinae Jiangxi

摘　　要：目的探讨过氧化氢-乙醚法制备脱蛋白骨(deproteinized bone,DPB)的理化性质。方法采用过氧化氢-乙醚法将大白兔干骺端松质骨脱脂、脱蛋白制作成生物衍生骨材料——DPB。倒置相差显微镜、体视学显微镜、扫描电子显微镜观察DPB材料外表面、孔隙内表面结构、孔径大小和孔间交通情况。pH计检测仪检测DPB材料的培养液pH值。光学显微镜观察微生物生长情况。微量凯氏定氮法检测DPB材料中蛋白质含量。结果倒置相差显微镜、体视学显微镜、扫描电子显微镜观察结果显示,DPB材料具有原骨组织的三维多孔-网架结构系统。计算机图像分析系统结果显示,孔隙率为(83.26±5.35)%;孔径最大径为(315.11±17.51)μm,最小径为(109.37±11.33)μm。浸泡DPB材料组培养液pH为7.383±0.015,空白对照组培养液pH为7.380±0.020。光学显微镜下观察结果显示,DPB材料的培养液未发现细菌或霉菌生长。DPB蛋白质含量为(20.4±1.2)%。结论由松质骨来源制作的DPB是一种较理想的组织工程骨生物支架材料。Objective To investigate the physical and chemical characters of deproteinized bone （DPB）processed with hydrogen dioxide and diethyl ether. Methods Metaphysic cancellous bone collected from adult white rabbit was digested with hydrogen peroxide and diethyl ether which converted the cancellous bone into DPB, a kind of biological derivative scaffold. The morphous of external and internal surface, porus diameter as well as transconnection of DPB was observed under inverted phase contrast microscope, stereomicroscope and scanning electromicroscope. The pH value of DPB culture solution was detected by pH counts. The microorganism development in culture solution was detected regularly by microscope. Applied Mirco-Kjeldahl method to detect the protein content of DPB. Results DPB processed by means of this procedure possessed threedimensional-pori-network-scaffold structure similar to original cancellous bone under inverted phase contrast microscope, stereomicroscope and scanning electromicroscope. The computer im age analysis system demonstrated that the porus diameter were（315.11± 17.51）μm maximal and （109.37±11.33）μm minimal and porosity was（83.26±5.35）%. The detected pH value of DPB culture solution was 7. 383 ±0. 015,and that of the control culture solution was 7. 380±0. 020. There was no bacterium and mould to be discovered in the DPB culture solution by microscopy.The detected protein content value was 20.4%±1.2 %. Conclusion The DPB derived from cancellous bone could perform a satisfactory role of bio-scaffold in tissue engineering bone.

关 键 词：脱蛋白骨 理化性质 过氧化氢 乙醚 

分 类 号：R68[医药卫生—骨科学] TQ932[医药卫生—外科学]