贞清胶囊对1型糖尿病大鼠周围神经病变改善作用的初步研究  

Amelioration effect of Zhenqing Capsule on peripheral neuropathy in type 1 diabetic rats

作　　者：文秀英[1] 胡勤锦[1] 王芳 

机构地区：[1]华中科技大学同济医学院梨园医院中西医结合科,湖北武汉430077 [2]湖南省马王堆老年病医院老年病科,湖南长沙421001

出　　处：《中西医结合学报》2008年第3期289-293,共5页Journal of Chinese Integrative Medicine

基　　金：国家自然科学基金(No.30672730)

摘　　要：目的:观察贞清胶囊对1型糖尿病大鼠周围神经病变的影响,并初步探讨其作用机制。方法:40只雄性Wistar大鼠,大剂量链脲佐菌素尾静脉注射造成1型糖尿病动物模型。成模大鼠随机分成3组:模型组、氨基胍组及贞清胶囊组,另设正常对照组。治疗10周后,观察治疗前后体质量和血糖变化以及治疗后血清丙二醛(malondialdehyde,MDA)和神经电生理变化,并留取坐骨神经标本做形态学观察。结果:贞清胶囊能显著降低1型糖尿病大鼠血清MDA水平(P<0.01);与模型组比较,贞清胶囊组的坐骨神经传导速度明显加快(P<0.05),潜伏期缩短(P<0.01),波幅增加(P<0.05);贞清胶囊治疗后坐骨神经髓鞘染色表现为部分神经纤维节段性脱髓鞘,与模型组的大部分神经纤维脱髓鞘相比有改善。结论:贞清胶囊能明显改善糖尿病大鼠的神经电生理功能,改善周围神经的病理变化,其机制有可能是通过清除自由基,抗脂质过氧化作用来实现。Objective： To investigate the effects of Zhenqing Capsule （ZQC）, a compound traditional Chinese herbal medicine, in treating type t diabetic rats with peripheral neuropathy. Methods. Type 1 diabetes was induced by caudal vein injection of high-dose streptozotocin in 30 male Wistar rats. The thirty diabetic rats were randomly divided into three groups： ZQC-treated group, untreated group and aminoguanidine-treated group. Another group of 10 rats was taken as normal control. After 10-week treatment, the changes of body weight and fasting plasma glucose level were measured, and the serum MDA level and the changes of neurological electrophysiology were analyzed. The samples of sciatic nerve in diabetic rats were taken for morphological observation. Results： The MDA level in type 1 diabetic rats was notably reduced in ZQC-treated group as compared with the untreated group （P〈0.01）. Compared with the untreated group, ZQC could improve the electrophysiology of sciatic nerve including conduction velocity （P〈0.05）. latency （P〈0.01） and wave amplitude （P〈0.05）. The nerve myelin staining results showed that segmental demyelination of the nerve fibers in ZQC-treated group was not as serious as that in the untreated group.Conclusion： ZQC can obviously ameliorate the neurological electrophysiological function and the pathological changes of peripheral nerve in type 1 diabetic rats through the removal of free radical and resistance of lipid peroxidation.

关 键 词：贞清胶囊 糖尿病 1型 糖尿病神经病变 丙二醛 大鼠 

分 类 号：R259[医药卫生—中西医结合]