扁蓿豆遗传多样性的SSR分析  

Genetic Diversity of Medicago ruthenica Based on SSR Markers

作　　者：李鸿雁[1] 米福贵[1] 宁红梅[1] 师文贵[2] 赵成贵 

机构地区：[1]内蒙古农业大学生态环境学院,内蒙古呼和浩特010010 [2]中国农业科学院草原研究所,内蒙古呼和浩特010010 [3]内蒙古凉城县畜牧局,内蒙古凉城013700

出　　处：《中国草地学报》2008年第2期34-38,共5页Chinese Journal of Grassland

基　　金：多年生牧草种质资源繁殖更新、鉴定评价与利用(NB05-070401-09)

摘　　要：应用SSR分子标记对内蒙古野生扁蓿豆4个地理种群进行了遗传多样性研究。结果表明,扁蓿豆具有较高的遗传多样性,12对引物共扩增出66个位点;物种水平上Nei's基因多样性指数为0.2222,Shannon多样性指数为0.3639,种内总基因多样性为0.2223;种群内基因多样性为0.2153,96.88%的遗传变异存在于种群内,3.12%的遗传变异存在于种群间;种群间的遗传分化系数为0.0312,基因流为15.5256;4个种群间有一定遗传分化,但遗传漂变不会引起遗传分化。UPGMA遗传距离聚类结果表明,生态地理条件相似的种群优先聚集。The genetic diversity of Medicago ruthenica, distributed in Inner Mongolia was analyzed using simple sequence repeat（SSR） markers. 66 bands were amplified from 4 natural populations by 12 reliable primers. A high level of genetic diversity was revealed. The Nei＇s gene diversity index was 0. 2222 and the Shannon diversity index was 0. 3639. The total gene diversity was 0. 2223, while the gene diversity within population was 0. 2153,96.88% of genetic variation existed within populations, while 3.12% of genetic variation existed among populations. The genetic differentiation coefficient was 0. 0312. The gene flow was 15. 5256. There was certain degree of differentiation among populations. However the genetic drift could not cause genetic differentiation, the UPGMA dendrogram indicated that populations with similar ecological and geographical conditions clustered first.

关 键 词：扁蓿豆 分子标记 SSR 遗传多样性 PCR 

分 类 号：S541[农业科学—作物学]