熏衣草微体快速繁殖与试管苗壮苗技术研究  

A Study on Techniques of Rapid Micro-propagation and Cultivating Strong Plantlets of Lavandula vera in Test Tube

作　　者：钟士传[1] 曹帮华[2] 

机构地区：[1]临沂师范学院,山东临沂276000 [2]山东农业大学,山东泰安271018

出　　处：《中国草地学报》2008年第2期116-120,共5页Chinese Journal of Grassland

基　　金：山东省农业良种工程项目计划(鲁科农字〔2006〕90号)

摘　　要：对熏衣草微体快速繁殖和壮苗技术进行了研究。适合于熏衣草试管苗繁殖的培养基为MS+6—BA2.0mg/L+NAA 0.05mg/L+蔗糖30g/L+琼脂7g/L,培养28d,繁殖系数达8以上;培养基中附加CCC质量浓度为0.05mg/L^0.5 mg/L时对熏衣草试管苗繁殖和壮苗有利;熏衣草试管苗生根用IAA的效果较好,适宜于生根的培养基配方为MS+IAA1.0mg/L+蔗糖20g/L+琼脂7g/L,培养25d生根率为91.6%;生根试管苗移栽成活率为73%。Rapid micro-propagation and cultivating strong plantlets of Lavandula vera were studied. The result showed that the optimum medium for micro-propagation of plantlet of Lavandula vera was MS＋ 6-BA 2.0mg/L ＋ NAA 0.05mg/L ＋ sucrose 30mg/L ＋ agar.7g/L, the propagation coefficient is above 8 after 25 days. The medium MS ＋ CCC 0.5mg/L - 0.5 mg/L, is favorable to cultivate strong seedlings. The medium MS ＋ IAA 1.0mg/L ＋ sucrose 20mg/L ＋ agar 7g/L is better for rooting, the rooting rate is 91.6% after 25 days. The survival rate of the transplant is 73%.

关 键 词：熏衣草 微体快速繁殖 植物生长调节剂 试管苗 壮苗培育 

分 类 号：Q813.1[生物学—生物工程]