缺氧诱导因子1α mRNA在肺出血新生鼠肺内的表达  

Expression of hypoxia inducible factor-1α mRNA in lungs of newborn rats with pulmonary hemorrhage

作　　者：陈玉君[1] 陈克正[2] 刘唐威[3] 

机构地区：[1]广西医科大学第一附属医院儿科重症监护病房,南宁530021 [2]广州市儿童医院新生儿重症监护病房 [3]广西医科大学第一附属医院心血管科

出　　处：《中国新生儿科杂志》2008年第2期92-94,共3页Chinese Journal of Neonatology

摘　　要：目的 探讨缺氧诱导因子1α mRNA（hypoxia inducible factor-1α mRNA，HIF-1仅mRNA）在新生鼠肺出血发生时肺内的表达。方法 选择生后7～8d的新生Wistar大鼠34只，通过低温缺氧后高氧复温建立肺出血模型，共设三组，分别为低温缺氧后高氧复温组（HHRO2）12只，给予缺氧和低温（0.5％～6％，10±1℃）6h，随后复温供氧（02〉95％，37℃=）2h。低温缺氧组（HH）12只，只缺氧和低温。正常对照组（C组）10只，置室温内不缺氧。各组取肺组织，观察大体、光镜下肺组织学改变，用逆转录．聚合酶链反应（RT-PCR）方法检测肺组织中HIF-1α mRNA表达。结果HHRO2组和HH组肺大体可见水肿和点状、局灶性甚至弥漫性出血；光镜下可见肺水肿、间隔断裂、肺泡扩大、肺泡融合及出血；HIF-1α mRNA在各组中表达差异无统计学意义（F=0．64，P〉0．05）。结论 在低温缺氧／复温供氧所致新生鼠肺出血发生过程中，低氧对HIF-1α的表达调控发生在转录后水平。Objective To investigate the expression of hypoxia inducible factor-lot mRNA （HIF- 1α mRNA） in pulmonary tissue of newborn rats with pulmonary hemorrhage. Methods Thirty-four newborn Wistar rats, 7 to 8 days old were randomly assigned into control group （group C, n = 10） and two experimental groups. Pulmonary hemorrhage was induced by hypoxia （5% -6% O2） and hypothermia （ 10 ± 1 ℃ ） for 6 hours. The experimental group was divided into only hypoxia and hypothermia （HH） group （n =12）, and HH followed by 37 ℃ 95% 02 group for2 hours （HHRO2, n =12）. The gross anatomical and histological changes identified by HE staining in lungs were observed. The expression of HIF-1 α mRNA was measured by RT-PCR. Results The lungs of rats in groups HH and HHRO2 presented edema and punctiform, local and diffused pulmonary hemorrhage. Histopathological changes included pulmonary edema, septal fragmentation, pulmonary alveoli dilatation, fusion of pulmonary alveoli and pulmonary hemorrhage. There was no significant difference in expressions of HIF-1α mRNA between group C and experimental groups （ F = 0.64, P 〉 0. 05 ）. Conclusion Hypoxia regulates the expression of HIF-1α by post-transcriptional mechanism when pulmonary hemorrhage induced by hypothermia/hy- poxia and re-warming/re-oxygenating occurs in newborn rats.

关 键 词：缺氧诱导因子1 MRNA 肺 

分 类 号：R722.1[医药卫生—儿科]