多元羧酸和磷酸对牙本质脱钙作用的比较  

Comparison of dentin decalcification between polycarboxylic acids and phosphoric acid

作　　者：沈晴昳[1] 傅柏平[2] 孙皎[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院,上海生物材料研究测试中心,上海市200023 [2]浙江大学医学院附属第一医院口腔科,浙江省杭州市310003

出　　处：《中国组织工程研究与临床康复》2008年第6期1055-1058,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:酸性较弱的多元羧酸对牙体硬组织具有化学吸附作用,故部分牙体黏接材料采用多元羧酸及其衍生物作为预处理剂。比较苹果酸、酒石酸、柠檬酸和传统酸蚀剂磷酸对牙本质的脱钙作用,分析多元羧酸的酸蚀能力。方法:实验于2006-10/2007-04在上海交通大学医学院附属第九人民医院和浙江大学医学院附属第一医院口腔科完成。采用150g/L苹果酸、150g/L酒石酸、150g/L柠檬酸溶液分别酸蚀牙本质60s,360g/L磷酸酸蚀牙本质15s,扫描电镜下观察牙本质处理前后表面形貌及牙本质小管直径,方差分析比较不同酸处理对牙本质小管直径大小的影响差异。结果:①扫描电镜下见未酸蚀样本的牙本质小管开口狭小。酸蚀处理后牙本质表面平坦、清晰,覆盖的玷污层基本去除,管周牙本质脱矿明显,牙质小管开口扩大、敞开。②不同种类酸溶液处理后牙本质脱矿,牙本质小管直径明显增大(P<0.01)。磷酸酸蚀15s、柠檬酸、酒石酸酸蚀60s的牙本质小管直径大小无差异(P>0.05),明显大于苹果酸酸蚀60s的小管直径(P<0.01)。结论:苹果酸的脱矿作用较弱,适当延长酒石酸、柠檬酸的处理时间可获得与磷酸酸蚀相当的牙本质脱矿效果。AIM： Polycarboxylic acids with poor acidity are chemical adhesive to the dental hard tissues, thus polycarboxylic acids and their derivates can be used as pretreating agents for dental adhesive materials. This study was designed to compare the dentin decalcification of three carboxylic acids （malic, tartaric and citric） with phosphoric acid. METHODS： The experiment was carried out in the Ninth People＇s Hospital, School of Medicine, Shanghai Jiao Tong University and the Department of Stomatology in the First Affiliated Hospital of Medical College, Zhejiang University from October 2006 to April 2007. Dentin was etched with 150 g/L malic acid, 150 g/L tartaric acid or 150 g/L citric acid solutions for 60 seconds, respectively, as well as 360 g/L phosphoric acid for 15 seconds. The surface morphology of dentin was observed with scanning electron microscopy （SEM） and the diameter of dentinal tubules was measured. The influences of different acids on the diameter of dentinal tubules were determined by using analysis of variance. RESULTS： 1.SEM results displayed that, the gap of dentinal tubules was narrow in the untreated dentins. After acid etching, the dentins were fiat, distinct and clean, with the absence of stains and the presence of peritubular dentin decalcification. The gap of dentinal tubules was enlarged.2.Tubular diameters were significantly increased after acid etching （P 〈 0.01）. There were no significant differences in the tubular diameter among phosphoric acid treatment for 15 seconds, citric acid for 60 seconds and tartaric acid for 60 seconds （P 〉 0.05）. And the tubule diameter of dentin treated by phosphate, citric or tartaric acids was significantly broader than that treated by malic acid （P 〈 0.01）. CONCLUSION： The demineralization capabilities of citric acid and tartaric acid to dentin are as powerful as phosphoric acid by increasing the application time, while the demineralization power of malic acid is much weaker.

关 键 词：羧酸 磷酸 牙本质小管 脱钙 生物材料 

分 类 号：R318.08[医药卫生—生物医学工程]