聚砜膜生物反应器实验系统构建及对重型肝炎患者血浆的影响(英文)  

Construction of polysulfone membrane bioreactor experimental system and its effects on plasma of patients with severe hepatitis

作　　者：陈志[1] 王英杰[2] 张世昌[2] 刘俊[2] 刘涛[2] 

机构地区：[1]解放军总医院第二附属医院全军结核病研究所,北京市100091 [2]解放军第三军医大学西南医院全军感染病研究所,重庆市400038

出　　处：《中国组织工程研究与临床康复》2008年第6期1175-1178,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30470458)~~

摘　　要：背景:人工肝反应器性能的评价通常需要构建一个密闭实验装置模拟生物人工肝支持系统来对反应器内肝细胞的生物功能以及生物反应效率等进行综合评价,对聚砜膜中空纤维反应器的性能进行初步评价对优化生物人工肝支持治疗系统具有一定意义。目的:构建聚砜膜生物反应器实验系统,了解该系统对重型肝炎患者血浆的影响,观察聚砜膜中空纤维反应器作为生物人工肝反应器的可行性。设计:重复测量实验。单位:第三军医大学西南医院全军感染病研究所。材料:选用7只新生1～7d中国实验小型猪,雌雄不拘,由第三军医大学实验动物中心提供,许可证号码:F99017实验过程中对动物的处置符合动物伦理学标准。肿瘤坏死因子α、转化生长因子β1试剂盒购自中国晶美生物工程公司;聚砜膜中空纤维反应器订制于中国上海德宏生物材料研究所;Cellco培养循环式人工毛细管培养系统购于美国Spectrum公司;7份重型肝炎患者血浆样本均取自住院慢性重型肝炎患者做血浆置换时分离的血浆。患者均对实验项目知情同意,实验经过医院伦理委员会批准。方法:实验于2004-01/2005-07在第三军医大学西南医院全军感染病研究所实验室完成。①实验过程:实验前12h对实验猪进行断乳,清洁皮毛后分离肝细胞。将聚砜膜中空纤维反应器的内腔通过氧和二氧化碳弥散管与培养液贮池和Cellco培养液循环式人工毛细管培养系统连接成为密闭系统,外腔接口用洁净橡胶塞封闭。用磁力搅动法将分离的肝细胞进行球形体培养,接种到本系统的聚砜膜中空纤维反应器外腔,然后加重型肝炎患者血浆样本200mL于贮液池中,以80mL/min的速度在反应器内腔及贮液池中循环。②实验评估:在循环的0,2,4,6h从反应器内腔各取出2mL循环液,用谷氨酸脱氢酶-紫外法检测上清中氨的水平,全自动生化分析仪上检测标本�BACKGROUND： Property of artificial liver reactor is generally evaluated by using an enclosed experimental device, which is constructed through simulating bioartificiai liver supporting system. Moreover, the enclosed experimental device is also comprehensively evaluated biological function of hepatocytes and biological response efficiency. In addition, it is significance for primarily evaluating the properties of polysuifone membrane hollow fiber reactor to optimize bioartificial liver supporting system. OBJECTIVE： To construct polysuifone membrane bioreactor experimental system, understand the effects on plasma of patients with severe hepatitis, and observe the feasibility of hollow fiber reactor regarded as bioartificial liver reactor. DESIGN： Repeated measurement. SETTING： General Infectious Disease Institute, Southwest Hospital, the Third Military Medical University of Chinese PLA. MATERIALS： Seven Chinese mini-pigs with 1-7 days old and of either gender were provided by Experimental Animal Center, the Third Military Medical University （certification： F99017）. The animal disposal accorded to the ethical standard. Tumor necrosis factor-α（TNF-α ） and transforming growth factor- β1 （TGF- β1） kits were purchased from Jingmei Bioengineering Company, China; polysuifone membrane hollow fiber reactor by Shanghai Dehong Biomaterial Institute, China; Ceiico culture-circular artificial capillary culture system by Spectrum Company, USA; seven plasma exponents were taken from admission patients with chronic severe hepatitis during plasma exchange. All patients provided the informed consent, and the animal experiment was conducted with confirmed consent by the local ethics committee. METHODS： This study was performed at the General Infectious Disease Institute, Southwest Hospital, the Third Military Medical University of Chinese PLA from January 2004 to July 2005. 1. Experimental procedure： The experimental pigs underwent ablactation at 12 hours before experiment, and then hepatocytes were se

关 键 词：聚砜膜 生物反应器 重型肝炎患者血浆 生物人工肝 猪肝细胞 

分 类 号：R318.08[医药卫生—生物医学工程]