生物衍生骨支架材料制备及在体内的组织相容性(英文)  

Preparation of bio-derived bone and its histocompatibility

作　　者：牛云[1] 何旭[1] 张丽红[1] 高婷[1] 许华[1] 李玉林[1] 

机构地区：[1]吉林大学基础医学院病理生物学教育部重点实验室,吉林省长春市130021

出　　处：《中国组织工程研究与临床康复》2008年第7期1385-1389,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家"八六三"重大专项(2004AA205020);教育部博士学科点专项科研基金(20020183064);国家自然科学基金(30700872)~~

摘　　要：背景:理想的支架材料必须对机体无毒性,无免疫排斥反应,并能适时地降解,具有良好的生物相容性,生物衍生骨支架材料能否长期存留体内并发挥功能值得研究。目的:观察生物衍生骨支架材料植入鼠体内后机体局部组织生物相容性及对免疫功能的影响。设计:随机对照动物实验。单位:吉林大学基础医学院病理生物学教育部重点实验室。材料:实验于2006-03/2006-07在吉林大学基础医学院病理生物学教育部重点实验室完成,选用18只雄性BALB/C小鼠,体质量(202)g,雌雄各半;1只昆明小鼠,体质量20g;1只雌性家兔,体质量2.5kg,以上实验动物均由吉林大学基础医院实验动物部提供,实验过程中对动物的处置符合动物伦理学标准。猪松质骨(髂骨)为市售。实验用IMDM培养基为美国Hycolone公司产品,FBS购自美国GIBCO公司,四甲基偶氮唑盐及ConA均为美国Sigma公司产品。方法:采用猪髂骨制备生物衍生骨支架材料。①采用随机抽签法将BALB/C小鼠分成支架材料植入组、异种骨植入组及对照组,每组6只。支架植入组将支架植入到BALB/C小鼠的下肢肌肉内,异种骨植入组将昆明鼠骨植入到BALB/C小鼠下肢肌肉内,对照组仅将局部肌肉损伤。②术后21d取材料植入部位及周围组织,大体及苏木精-伊红染色法进行观察材料与组织相容性;采用刀豆蛋白A诱导各组脾淋巴细胞转化,酶联免疫检测仪在λ570nm波段检测并记录淋巴细胞刺激指数。以补体依赖性细胞毒实验评估各组小鼠免疫功能,即采用酶联免疫检测仪在λ570nm波段检测并记录各组吸光度值,计算细胞杀伤率。主要检测指标:①植入物周围组织相容性。②刀豆蛋白A诱导后淋巴细胞刺激指数。③补体依赖性细胞毒实验各组细胞杀伤率。结果:18只BALB/C小鼠均进入结果分析。①植入物周围组织相容性:支架材料植入组植入21d后,支架材料组肉眼可见,埋植�BACKGROUND： The ideal biomaterial means absence of cytotoxic effect and immunological rejection, degradation at right moment, and a well histocompatibility. Whether bio-derived bone can be used in vivo for long time and exerts functions deserves to be studied. OBJECTIVE： To investigate the local histocompatibility after bio-derived bone implanted into mouse and the effect on immunofunctions. DESIGN： A randomized controlled animal experiment. SETTING： Key Laboratory of Pathobiology, Ministry of Education, School of Basic Medical Sciences, Jilin University. MATERIALS： This study was performed at the Key Laboratory of Pathobiology, Ministry of Science, School of Basic Medical Sciences, Jilin University from March to July in 2006. Eighteen BALB/C mice （weighing 20±2 g, half male and half female）, one male Kunming mouse （weighing 20 g）, and one female rabbit （weighing 2.5 kg） were included in the experiment. All the experimental animals were provided by Laboratory Animal Center, School of Basic Medical Science, Jilin University. The disposal of the experimental animals in the test process accorded with ethical guidelines for the use and care of animals. Porcine cancellous bone （iliac bone） was purchased from the market. Iscove＇s Modified Dulbecco＇s Medium （IMDM, Hycolone, USA）, fetal bovine serum （FBS, Gibco Co., Ltd, USA）, methyl thiazolyl tetrazolium （MTT, Sigma, USA ） , and concanavalin A （ConA, Sigma Co., Ltd, USA） were used. METHODS： Bio-derived bone was prepared from commercial porcine bone. ① Eighteen BALB/C mice were randomly divided into three groups with 6 mice in each group： a control group （simple local muscle injury without implantation）, a bio-derived bone implantation group （ implanting bio-derived bone into the lower limb）, and a xenogenic bone implantation group （femoral bone from Kunming mouse was implanted into the muscle of lower limb）. ② Twenty-one days after operation, the implant material and surrounding tissue were obt

关 键 词：生物衍生骨支架 组织相容性 骨组织工程 组织构建 

分 类 号：R318[医药卫生—生物医学工程]