糖尿病大鼠瘦素和下丘脑瘦素功能受体的表达与胃排空的关系  

The relationship between gastric emptying and expression of Leptin in diabetic rats

作　　者：于卫星[1] 魏良洲[1] 李新华[1] 田字彬[1] 杨林[1] 

机构地区：[1]青岛大学医学院附属医院消化科,266003

出　　处：《中国临床实用医学》2008年第3期10-12,共3页China Clinical Practical Medicine

摘　　要：目的探讨糖尿病不同阶段胃排空障碍与血清瘦素及其下丘脑OB-Rb表达的关系。方法40只Wistar大鼠随机分为对照饲养1周组（NC1组；n=6）、对照饲养4周组（NC2组；n=6）、糖尿病造模1周组（DM1组；n=14）和糖尿病造模4周组（DM2组；n=14）。分别于给药后1周、4周用酚红灌胃法检测胃排空；用放射免疫法、半定量RT—PCR技术检测大鼠血清瘦素及其下丘脑OB-Rb mRNA的表达。结果注射STZ1周后，DM1组大鼠血清瘦素明显低于NC1组（P〈0．05）；下丘脑OB-Rb mRNA表达明显低于NC1组；液体胃排空是加快的（P〈0．01）。注射STZ4周后，DM2组大鼠血清瘦素和胃排空明显低于NC2组（P〈0．05；P〈0．01）；DM2组下丘脑组织OB—Rb mRNA的表达明显高于NC2组（P〈0．05）。结论Ⅰ型糖尿病早期，机体可能通过降低血清瘦素和反应性的下调下丘脑OB-Rb表达，以加快胃排空。维持能量平衡。而长期高血糖及低瘦素水平可能反馈性的引起下丘脑OB-Rb表达上调，参与胃排空延迟。Objective Aim To explore the relationship between gastric emptying and expression of leptin and its receptor in different diabetic stages. Methods Fourty Wistar rats were randomly divided into four groups ： 1-week normal control group（ NC1 group ; n = 6 ）, 4-week normal control group （ NC2 group ; n = 6 ） , 1 - week diabetes mellitus group（ DM1 group;n = 14）and 4-week diabetes mellitus group（ DM2 group;n = 14）. One week and four weeks after the STZ injection,gastric emptying measured by intragastric administration of phenol red;serum leptin concentrations were measured with that of radioimmunoassay; expression of OB-Rb mRNA in hypothalamus was detected with semi-quantitative RT-PCR. Results 1-week after the STZ injection,the expression of serum leptin and OB-Rb mRNA in hypothalamus of DM1 group was decreased significantly compared with that of the control（ P 〈 0. 05 ; P 〈 0.05 ） ; DM1 group showed significantly accelerated rates of gastric emptying of the fluid compared with that of the NC1 group（ P 〈 0. 01 ）. 4-week after the STZ injection, serum leptin and gastric emptying of the DM2 group decreased significantly compared with that of the NC2 group（ P 〈 0. 05 ; P〈 0. 01 ）. Oppositely, the expression of OB-Rb mRNA in the hypothalamus of DM2 group increased significantly（P 〈0.05）. Conclusion The decreased serum leptin and OB-Rb in the hypothalamus on the short-term hyperglycemia may participate in the rapid gastric emptying and increase ingestion reaction, which can maintain energy balance of the body. The high expression of OB-Rb in the hypothalamus may be a protective mechanism that it could increase leptin sensitivity to inhibit gastric emptying on the long-term hyperglycemia.

关 键 词：糖尿病 胃排空 瘦素 OB—Rb 

分 类 号：R285.5[医药卫生—中药学]