联合检测抗环瓜氨酸肽抗体与抗角蛋白抗体对RA诊断意义  

Significance of Combined Detection of Anti-cyclic Citrullinated Peptide Antibody and Anti-keratin Anti-body in the Diagnosis of Rheumatoid Arthritis

作　　者：孙善会[1] 郑燕[1] 黄平[1] 汪运山[1] 

机构地区：[1]山东大学附属济南市中心医院医学实验诊断中心,250013

出　　处：《中国临床实用医学》2008年第3期29-30,共2页China Clinical Practical Medicine

摘　　要：目的联合检测抗CCP抗体和AKA，评价血清学临床诊断意义。方法对320例有症状性关节痛或关节炎的患者，ELISA法检测抗CCP IgG抗体，间接免疫荧光法检测AKA，ELISA法检测RF IgA、IgG、IgM。结果抗CCP抗体、AKA、RF的阳性率分别为25．9％（83／320）、30．3％（97／320）、43．4％（139／320），30例正常对照中，仅2例RF阳性，1例CCP阳性，其余指标均为阴性。三种抗体同时阳性率23．8％（76／320），明显高于正常对照组（P〈0．01）。结论抗CCP抗体和AKA并同时检测RF IgA、IgG、IgM。有助于诊断和早期诊断。并可作为临床治疗动态观察指标。Objective To assess the significance of combined detection of anti-cyclic citrullinated peptide antibody and anti-keratin antibody in the diagnosis of rheumatoid arthritis. Methods A total of there hundred and twenty patients with established RA was enrolled. Serum anti-cyclic citrullinated peptide（ CCP）anti- bodies and IgA, IgGand IgM rheumatoid factor（RF） were measured by enzyme-linked immunosorbent assay （ELISA）, Anti-keratin antibody（ AKA）was detected by indirect immunofluorescence assay using rat esophagus as substrate. Results The prevalence of various autoantibodies was： IgM RF 82. 2% , anti-CCP antibodies 30. 3% ,AKA 25.9% ,and RF 43.4%. All but one healthy control sample were positive for AKA and two were positive for RF. 76 of the 320 patients（23.3 % ） were positive for the three kinds of antibodies and the presence was significantly higher than the healthy control group（P 〈 0.01 ）. Conclusion The combined detection of anti-CCP and AKA,in addition to RF was useful in establishing early diagnosis of RA, and can be a useful parameter in the clinical therapy.

关 键 词：抗环瓜氨酸肽抗体 抗角蛋白抗体 类风湿因子 类风湿关节炎 

分 类 号：R593.22[医药卫生—内科学] R441[医药卫生—临床医学]