孕妇血中胎儿DNA荧光定量PCR检测及与临产关系  

FETAL DNA IN MATERNAL PLASMA AND ITS RELATION WITH PARTURIENT PERIOD

作　　者：张怡梅[1] 柳林[2] 王修海[1] 

机构地区：[1]青岛大学医学院生物学教研室,山东青岛266021 [2]潍坊市人民医院妇产科

出　　处：《青岛大学医学院学报》2008年第1期32-34,共3页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：目的探讨晚孕期和临产后母血中胎儿DNA含量的变化及其临床意义。方法运用实时荧光定量PCR技术分别检测孕28-42周以及临产时孕妇血浆中胎儿DNA的含量并进行比较。结果孕28-31周、孕32-35周、孕36-42周和临产时的母血浆中胎儿DNA的平均含量比较差异有显著性（F=308.19,q=11.59-40.33,P〈0.01）。结论随着孕期的进展,母血中胎儿DNA含量的升高可能是分娩的诱因之一。Objective To study the changes of content of fetal DNA in maternal plasma in late pregnancy and in labor, and explore their significance. Methods The content of fetal DNA in maternal plasma in 28-42 weeks of gestation and parturient period were detected by using real-time quantitative PCR technique. Results The differences of mean content of fetal DNA in maternal plasma among 28-31-week, 32-3S-week, and 36-42-week gestation and parturient period were significant （F= 308.19,q=ll. 59-40.33,P〈0.01）. Conclusion With the progress of duration of pregnancy, the fetal DNA in maternal blood increases, which is, most likely, one of the inducements leading to delivery.

关 键 词：孕妇 胎儿 DNA 聚合酶链反应 

分 类 号：R714.2[医药卫生—妇产科学]