新疆雪莲体外直接器官发生的快繁研究  

Research on Rapid Micro-propagation Via Direct Organogenesis of Saussurea involucrata Kar.et Kir.

作　　者：傅晓春[1] 王晓军[1] 赵民安[1] 刘敏[1] 赵海清[1] 贾莉芳[1] 

机构地区：[1]中国科学院新疆理化技术研究所,新疆乌鲁木齐830011

出　　处：《干旱区研究》2008年第2期248-253,共6页Arid Zone Research

基　　金：中国科学院西部行动高新技术项目:新疆雪莲规模化组培快繁技术研究(KGCX2-SW-506)

摘　　要：以新疆雪莲无菌苗的下胚轴、子叶、真叶和根为外植体,在MS基本培养基上培养,探索新疆雪莲体外直接器官发生的快繁途径。结果表明:新疆雪莲不同外植体直接再生不定芽的效果不同,以真叶为外植体,直接再生芽频率最高,在MS+6-BA1.0 mg/L(单位下同)+NAA0.1+2,4-D0.1上,30 d芽诱导率可达66%;在MS+6-BA0.5+NAA0.05上,芽的增殖生长效果好;在1/2MS+NAA0.1上,25 d内生根率达84.7%;在珍珠岩∶腐叶土为1∶3的基质中炼苗,成活率达89.1%。This study researches the rapid micro-propagation ofSaussurea involucrata Kar. et Kir. with adventi- tious buds induced from hypocotyls, cotyledons, leaves, and roots by applying Murashige-Skoog （MS） as the basic medium. The effects of different kinds of external hormones and explants on the rapid micro-propagation through direct organogenesis are explored. The experimental results show that the efficiencies of the direct organogenesis and budding regeneration are different from different explants, and the direct budding frequency is the highest when leaves are used as the explants. The induced budding ratio can be as high as 66% during 30 days in MS medium supplemented with 6-benzyladenine （6-BA） of 1.0 mg/L and α-naphthalene acetic acid （NAA） of 0.1 mg/L, and in 2,4-Dichlorophenoxyacetic acid （2,4-D） of 0.1 mg/L; the budding multiplication is quick and ideal in MS medium supplemented with 6-BA of 0.5 mg/L and NAA of 0.05 mg/L, and the induced rooting ratio can be as high as 84.7% during 25 days. If the sprouts are transplanted into the substrate containing perlite and humus soil

关 键 词：新疆雪莲Saussurea INVOLUCRATA 直接器官发生 无菌苗 快速繁殖 

分 类 号：S567.239[农业科学—中草药栽培]