壬基酚对小鼠睾丸芳香化酶表达的影响  

Effect of nolyphenol on the expression of aromatizing enzyme in mice testis

作　　者：周云国[1] 路军秀[2] 徐国峰[1] 薛利敏[1] 孙娟[1] 袁向山[2] 马全祥[2] 

机构地区：[1]新乡医学院基础医学院,2004级河南新乡453003 [2]新乡医学院组织胚胎学教研室,河南新乡453003

出　　处：《新乡医学院学报》2008年第2期180-182,共3页Journal of Xinxiang Medical University

基　　金：河南省教育厅自然科学研究计划项目(编号:2007310009);新乡医学院科研资助项目(编号:2005YJ07);新乡医学院大学生科研资助项目

摘　　要：目的研究壬基酚(NP)对雄性小鼠睾丸组织中芳香化酶表达的影响。方法健康雄性昆明小鼠30只,随机分为对照组、1%NP组和1‰NP组。10d后处死各组小鼠,取出睾丸,Zamboni固定液固定,常规冰冻制片,免疫组织化学染色,光镜观察。结果对照组小鼠睾丸组织中芳香化酶呈强阳性表达,1%NP组和1‰NP组小鼠睾丸组织中芳香化酶阳性表达明显减弱(P<0.01),1%NP组和1‰NP组间无明显差异(P>0.05)。结论NP可导致小鼠睾丸组织中芳香化酶表达降低。Objective To study the effect of nolyphenol on the expression of aromatizing enzyme in testis of male mice. Methods Thirty healthy male mice were randomly divide into control group,1% nolyphenol group and 1‰ nolyphenol group. On the 10th day,quickly took out testis of mice, fix in the Zamboni fixation solution, conventionally produce frozen section and stain section by immunohistochemical technology. Results The expression of aromatizing enzyme was positive obviously in control group, and the positive expression attenuated obviously in 1% nolyphenol group and 1‰ nolyphenol group（P 〈0. 01 ） ,but there was no diffetent between 1% nolyphenol group and 1‰ nolyphenol group（P 〉0.05）. Conclusion Nolyphenol can reduce the expression of aromatizing enzyme in testis of mice. After mice was fed nolyphenol ,the expression of aromatizing enzyme visibly reduced.

关 键 词：壬基酚 芳香化酶 睾丸 小鼠 

分 类 号：R994.6[医药卫生—毒理学]