激素配比对黄瓜子叶再生和抗氧化酶活性的影响  

Effect of Hormone Combination on Cucumber Cotyledon Regeneration and Antioxidase Activity

作　　者：朱妍妍[1] 张卫华[2] 于玉梅[3] 王志峰[2] 曹齐卫[2] 王秀峰[1] 孙小镭[2] 

机构地区：[1]山东农业大学园艺科学与工程学院,山东泰安271018 [2]山东省农业科学院蔬菜研究所,山东济南250100 [3]湖南农业大学园艺园林学院,湖南长沙410128

出　　处：《山东农业科学》2008年第2期36-38,共3页Shandong Agricultural Sciences

基　　金：山东省中青年科学家科研奖励基金;山东省农业科学院创新基金(2007YCX022);山东省农业科学院博士基金(2006YBS008);"863"计划(2006AA100108-3-1)资助

摘　　要：在建立和优化黄瓜子叶不定芽再生系统的基础上,研究了黄瓜再生过程中激素配比对黄瓜再生频率和抗氧化酶SOD、POD和CAT活性变化的影响。试验表明:激素ABA和Ades对黄瓜不定芽的再生频率的影响很大,其中在MS+6-BA 1 mg/L+ABA 1 mg/L+Ades 40 mg/L培养基上的出芽率最高,达90.0%;在培养早期ABA和Ades都能明显提高外植体SOD含量,降低POD含量;黄瓜不定芽再生与SOD含量呈正相关,与POD含量呈负相关。After a high efficient cucumber cotyledon regeneration system was established, the effect of hormone combination on the activities of SOD, CAT and POD were studied during the cucumber regeneration from cotyledons. The results indicated that ABA and Ades could notably increase the cucumber regeneration frequency ; the generation rate was the highest in the medium MS ＋ 6-BA 1 mg/L ＋ ABA 1 mg/L ＋ Ades 40 mg/L. ABA and Ades could also increase the content of SOD in the explants, at the meanwhile decrease the content of POD during the early stage of cucumber regeneration ; SOD had positive while POD had negative relation to cucumber regenetation.

关 键 词：黄瓜 不定芽诱导 激素配比 抗氧化酶活性 

分 类 号：S642.243[农业科学—蔬菜学]