金线莲种子培养的研究  

Seeds Culture of Anoectochilus roxburghii

作　　者：伍成厚[1] 冯毅敏[1] 贺漫媚[1] 陈妙贤[1] 朱纯[1] 叶振华[1] 

机构地区：[1]广州市园林科学研究所

出　　处：《中国野生植物资源》2008年第1期47-50,共4页Chinese Wild Plant Resources

基　　金：广东省科技计划项目(2004B60302006);广州市科技计划项目(2004Z3-E0361);广州市建委项目[(2003)06]

摘　　要：金线莲种子在培养基1/2MS+6-BA1.0 mg/L+NAA1.0 mg/L上萌发后形成原球茎,原球茎可以直接发育成幼苗,也可以由原球茎产生愈伤组织,再由愈伤组织发育成类原球茎而分化成幼苗。通过类原球茎可以实现大量增殖,在MS+6-BA1.0 mg/L+NAA0.1 mg/L上培养60 d的增殖倍数达到6.7倍。在培养基MS+IBA0.3mg/L上,金线莲的生根率可达到96.0%。Seeds of Anoectochilus roxburghii would germinate and develop to protocorms on the medium of 1/2MS＋6-BA1.0 mg/L ＋ NAA1.0 mg/L. The protocorms may develop to seedlings immediately or induce callous and then induce protocorm-liked-bodies which would develop to seedlings also. Anoec-tochilus roxburghii be rapidly propagated by the later method and 6.7 folds of propagation would get on the medium of MS＋6-BA1.0 mg/L＋ NAA0.1 mg/L after subcultured 60 days. Seedling＇s rooting rate was 96.0% on the medium of MS＋IBA0.3 mg/L.

关 键 词：金线莲 种子 原球茎 类原球茎 组织培养 

分 类 号：Q943.1[生物学—植物学]