丹参DNA提取方法比较  

Comparison of Genomic DNA Extraction Methods for Salvia miltiorrhiza

作　　者：宋振巧[1] 王明明[1] 王建华[1] 王洪刚[1] 

机构地区：[1]山东农业大学农学院,山东泰安271018

出　　处：《中国野生植物资源》2008年第1期54-57,共4页Chinese Wild Plant Resources

基　　金：2006年度山东省农业良种工程重大课题-大宗道地中药材(丹参等)优良品种筛选

摘　　要：为从富含多糖、酚类物质的丹参幼叶中提取高质量的核总DNA,比较了4种DNA提取方法对丹参叶片的提取效果,结果表明:改良CTAB法提取的DNA溶液颜色为无色透明、A260/A230为2.137、A260/A280为1.816值最好,是提取丹参基因组DNA的有效方法。To get high-quality DNA from young leaves of Salvia miltiorrhiza which consist of polysaccha-rides and phenols, four different DNA-extracting methods were compared in this paper. The results showed that： The improved CTAB was the best method since the DNA extracted by it had the best color of liquor, A260/A230 ratio and A260/A280 ratio.

关 键 词：丹参 SDS CTAB法 

分 类 号：Q523[生物学—生物化学]