清肠栓对大鼠溃疡性结肠炎caspase-3影响的研究  

Effect of Qingchang Suppository on the expression of caspase-3 in an experimental model of ulcerative colitis

作　　者：谢建群[1] 张涛[2] 郑昱[1] 马贵同[1] 陆雄[1] 薛筠[1] 施斌[1] 徐海珍[1] 袁建业[1] 

机构地区：[1]上海中医药大学附属龙华医院,上海201203 [2]浙江省中西医结合医院,浙江杭州310003

出　　处：《中成药》2008年第3期340-343,共4页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金(No.30772801)

摘　　要：目的:观察大鼠溃疡性结肠炎结肠黏膜固有层淋巴细胞凋亡及其凋亡调控关键酶caspase-3的表达以及清肠栓对相关表达的影响,探讨清肠栓(马齿苋、青黛、三七、五倍子)治疗溃疡病性结肠炎的作用机制。方法:64只SD大鼠随机分为4组,分别为正常组、模型组、SASP组、清肠栓组,每组各16只。除正常组外,其余大鼠用5%2,4,6-三硝基苯磺酸100 mg/kg灌肠建立大鼠溃疡性结肠炎模型后,分别予0.9%生理盐水、SASP、清肠栓处理7 d后,处死大鼠。运用电镜,免疫组化染色、RT-PCR、Western-blot等技术,分别检测溃疡性结肠炎大鼠结肠黏膜固有层淋巴细胞凋亡及固有层淋巴细胞caspase-3的表达。结果:清肠栓组结肠黏膜固有层淋巴细胞凋亡呈上升趋势;其结肠黏膜固有层淋巴细胞上caspase-3的表达率上升,与模型蛆相比(P<0.01)。结论:清肠栓可能增加结肠黏膜淋巴细胞凋亡效应酶caspase-3表达,诱导结肠黏膜固有层淋巴细胞凋亡,降低淋巴细胞毒作用,缓解大鼠溃疡性结肠炎效应。AIM ： To observe the lymphocyte apoptosis of colon mucosal lamina propria and the expression of its regulatory proteins caspase-3 in an experimental models of ulcerative colitis in rats as well as to survey the effect of Qingchang Suppository （ Herba Portulacae, Indigo naturalis, Radix et Rhizoma notoginseng, Galla Chinensis） on intestine mucosa in ulcerative colitis. METHODS： 64SD rats were randomly divided into 4 groups, each group consisted of sixteen animals, group （ 1 ） ： normal control ; group （2） ： blank ; group （3） ： SASP solution ; group （4） ： Qingchang Suppository. The rat model of ulcerative colitis was brought about by an enema of 100 mg/kg of TNBS. Beginning from the third day, except for the normal group, each group was treated with an enema of 0.9% NaC1, SASP, Qingchang Suppository respectively. The rats were sacrificed 1 week later. Apoptosis of lymphocyte was detected with the technique of transmission electron microscope （ H600 ）, While caspase-3 proteins expressions were assayed with immunohistochemical staining,reverse-transcript PCR, Western-blot. RESULTS ： Qingchang Suppository group showed higher rates of apoptosis than the blank controls. Rates of caspase-3 expression cells in LP were higher in UC than its expression in the controls （ P 〈 0.01 ）. CONCLUSION ： Qingchang Suppository showed to exert treatment effect on the rat model of ulcerative colitis possibly by promoting the expression of caspase-3 on UC colonic LP as well as increasing the apoptosis of LPL.

关 键 词：清肠栓 溃疡性结肠炎 结肠黏膜固有层淋巴细胞凋亡 半胱氨酸天冬氨酸酶一3 

分 类 号：R944.27[医药卫生—药剂学]