扶正抑瘤颗粒对小鼠移植性肿瘤细胞间通讯的影响  

Effect of Fuzheng Yiliu Granule on transplanted turmor gap junctional intercellular communication in mice

作　　者：王学习[1] 赵健雄[1] 陈茹[1] 程卫东[1] 白德成[2] 

机构地区：[1]兰州大学中西医结合研究所,甘肃兰州730000 [2]兰州大学医学实验中心,甘肃兰州730000

出　　处：《中成药》2008年第3期343-346,共4页Chinese Traditional Patent Medicine

基　　金：教育部科学技术研究重点项目(No.01128)

摘　　要：目的:观察扶正抑瘤颗粒(红芪、当归、墓头回、莪术)含药血清对荷瘤小鼠H22细胞的凋亡和肿瘤细胞间通讯的影响。方法:Annexin V/PI双染法检测FYK含药血清对H22细胞凋亡情况的影响;应用共聚焦激光显微镜检测H22细胞RNA、DNA、[Ca2+]、细胞间通讯状况。结果:扶正抑瘤颗粒含药血清可引起H22细胞的凋亡,细胞RNA、DNA含量降低,[Ca2+]升高,细胞间通讯状况得到改善。结论:扶正抑瘤颗粒含药血清抑制H22细胞的增殖,并提高肿瘤细胞[Ca2+],阻止肿瘤细胞分裂。ABSTRACT： AIM ： To investigate the apoptotic effect of Fuzhenyuliu Granules （FYG） （ Radix hedysari, Radix angelicae Sinesis, Rhizoma cucumae） drug-serum on gap junctional intercellular communication of murine transplanted hepatoma cell lines H22 in vitro, METHODS：Annexin V/PI double staining was used to observe the apoptosis of hepatoma cell lines H22. Confocal laser-scanning microscope to analyze the change in gap junctional intercellular communication. RESULTS ： FYG could enhance the apoptosis rate and Ca^2＋ concentration of hepatoma cells, reduce DNA and RNA content and improve gap junctional intercellular communication of H22 cell in vitro. CONCLUSION： FYG can inhibit the transplanted hepatoma prolification and increase hepatoma cell calcium concentration to stop hepatoma cell divison.

关 键 词：扶正抑瘤颗粒 中药血清 [Ca^2+] 细胞间通讯 

分 类 号：R285.6[医药卫生—中药学]